Antibiotic resistance in an in vitro subgingival biofilm model
Antibiotic resistance in an in vitro subgingival biofilm model
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DOI：
10.1111/j.1399-302x.2007.00366.x
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发表时间：
2007-10-01
影响因子：
--
通讯作者：
Walker, C.
中科院分区：
文献类型：
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作者：
Sedlacek, M. J.;Walker, C.
简介：本研究的目的是利用龈下菌斑的体外生物膜模型来研究龈下生物膜群落对牙周治疗常用抗生素的耐药性。生物膜在胰蛋白酶-大豆肉汤中在唾液包被的羟基磷灰石载体上生长4小时-10天，然后暴露于增加两倍浓度的四环素、阿莫西林、克林霉素，和红霉素或治疗上可达到的浓度的四环素，强力霉素，米诺环素，阿莫西林，甲硝唑，阿莫西林/克拉维酸，和阿莫西林/metronidazol.Results：抑制细菌菌株在稳态生物膜中所需的浓度是抑制相同的菌株生长所需的浓度的250倍。在治疗上可用的抗生素浓度的存在下，当生物膜达到稳态生长时，显著更高比例的生物膜保持存活。阿莫西林/克拉维酸和阿莫西林/甲硝唑的组合在抑制生长方面最有效。这些组合对长达并包括7天龄的生物膜特别有效，并且相对于未处理的对照抑制了90%或更多的细菌。由于生物膜接近稳定状态，这些组合是不太有效的50-60%的细菌保留violence.Conclusion：大多数，但不是全部，种龈下细菌是相当多的耐生物膜比在牙周文化。耐药性似乎与年龄有关，因为生物膜表现出渐进的抗生素耐药性，随着它们的成熟，最大耐药性与生物膜生长的稳态阶段一致。
Introduction: The purpose of this study was to utilize an in vitro biofilm model of subgingival plaque to investigate resistances in subgingival biofilm communities to antibiotics commonly used as adjuncts to periodontal therapy.Methods: Biofilms were grown on saliva-coated hydroxyapatite supports in trypticase-soy broth for 4 h-10 days and then exposed for 48 h to either increasing twofold concentrations of tetracycline, amoxicillin, clindamycin, and erythromycin or therapeutically achievable concentrations of tetracycline, doxycycline, minocycline, amoxicillin, metronidazole, amoxicillin/clavulanate, and amoxicillin/metronidazole.Results: Concentrations necessary to inhibit bacterial strains in steady-state biofilms were up to 250 times greater than the concentrations needed to inhibit the same strains grown planktonically. In the presence of therapeutically available antibiotic concentrations, significantly higher proportions of the biofilms remained viable as the biofilms reached steady-state growth. The combinations of amoxicillin/clavulanate and amoxicillin/metronidazole were the most effective in suppressing growth. These combinations were particularly effective against biofilms up to and including 7 days of age and inhibited 90% or more of the bacteria present relative to untreated controls. As the biofilms approached steady state, these combinations were less effective with 50-60% of the bacteria retaining viability.Conclusion: Most, but not all, species of subgingival bacteria are considerably more resistant in biofilms than in planktonic cultures. Resistance appeared to be age-related because biofilms demonstrated progressive antibiotic resistance as they matured with maximum resistance coinciding with the steady-state phase of biofilm growth.