A two-dimensional polarization interferometry based parallel scan angular surface plasmon resonance biosensor
A two-dimensional polarization interferometry based parallel scan angular surface plasmon resonance biosensor
复制标题
DOI：
10.1063/1.3553028
复制
发表时间：
2011-02-01
影响因子：
1.6
通讯作者：
Guo, Jihua
中科院分区：
文献类型：
--
作者：
Liu, Le;Ma, Suihua;Guo, Jihua
提出了一种基于二维偏振干涉的平行扫描角表面等离子体共振（SPR）生物传感技术。线形光照明和平行扫描的方法提供了高吞吐量。SPR角谱的同时记录使系统不受光源随时间变化的影响。偏振干涉技术降低了表面等离子体共振下降的最小值，从而减少了与光强相关的噪声。在我们的角度询问传感器中，在正常条件下的折射率分辨率为1.4 × 10（-6）折射率单位（RIU），在更耗时的条件下的折射率分辨率为4.6 × 10（-7）RIU。同时，人工制备的DNA微阵列已被检测，显示该技术在微阵列分析中的潜在应用。(C)2011年美国物理学会。[doi：10.1063/1.3553028]
We describe a two-dimensional polarization interferometry based parallel scan angular surface plasmon resonance (SPR) biosensing technique. The method of line-shaped light illumination and parallel scan offers a high throughput. The simultaneous record of SPR angular spectrum enables the system to be unaffected by the time-dependent variation of the light source. The polarization interferometry technique lowers the minimum of the SPR dip and thereby reduces the noise related to the light intensity. Refractive index resolutions of 1.4 x 10(-6) refractive index unit (RIU) under normal condition and 4.6 x 10(-7) RIU under a more time-consuming condition are achieved in our angle interrogation based sensor. Meanwhile, a manually prepared DNA microarray has been detected, showing the potential applications of this technique in microarray analysis. (C) 2011 American Institute of Physics. [doi:10.1063/1.3553028]