ISLET CELL AND EXTRAPANCREATIC EXPRESSION OF THE LIM DOMAIN HOMEOBOX GENE ISL-1
ISLET CELL AND EXTRAPANCREATIC EXPRESSION OF THE LIM DOMAIN HOMEOBOX GENE ISL-1
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DOI：
10.1210/mend-5-11-1633
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发表时间：
1991-11-01
影响因子：
--
通讯作者：
DRUCKER, DJ
中科院分区：
文献类型：
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作者：
DONG, JL;ASA, SL;DRUCKER, DJ
朗格汉斯胰岛的表型不同的细胞类型以一种受调控的和细胞特异性的方式表达许多不同的激素基因。对控制胰岛素基因转录的重要分子因素的分析导致了一些胰岛素基因结合蛋白的鉴定。其中一些蛋白质的cDNA已经被分离出来，包括ISL-1，据报道在胰岛细胞中优先表达的是LIM结构域的同源框蛋白。我们现在报道，ISL-1mRNA转录本不仅存在于胰岛细胞中，而且存在于嗜铬细胞瘤(PC-12)细胞系中。ISL-1mRNA转录本也在大鼠大脑的几个不同区域以及大鼠肾脏中检测到。对大鼠胰岛细胞系和人胰腺内分泌肿瘤的基因表达模式的分析表明，肿瘤的激素表型与ISL-1和胰岛素基因的表达存在不同的关系。免疫细胞化学研究表明，ISL-1在人胰腺内分泌肿瘤、正常胰腺A、B、D和PP细胞以及肾脏远端肾小管上皮细胞中均有胞浆和核染色。ISL-1免疫反应阳性神经元也广泛分布于中枢和外周神经系统、垂体以及一些人的垂体瘤和嗜铬细胞瘤。这些研究的结果暗示了ISL-1在内分泌胰腺外在神经系统和肾脏中的潜在作用。
The phenotypically distinct cell types of the islets of Langerhans express a number of different hormone genes in a regulated and cell-specific manner. Analysis of the molecular factors important for the control of insulin gene transcription has led to the identification of a number of insulin gene-binding proteins. The cDNAs for several of these proteins have been isolated, including isl-1, a LIM domain homeobox protein reported to be preferentially expressed in islet cells. We now report that isl-1 mRNA transcripts are present not only in islet cells, but also in pheochromocytoma (PC-12) cell lines. Isl-1 mRNA transcripts were also detected in several different regions of rat brain as well as in rat kidney. Analysis of the patterns of gene expression in rat islet cell lines and human pancreatic endocrine tumors demonstrated a variable relationship between the hormonal phenotype of the tumor and expression of the isl-1 and insulin genes. Immunocytochemical studies demonstrated both cytoplasmic and nuclear staining for isl-1 in human pancreatic endocrine tumors, in normal pancreatic A, B, D, and PP cells, and in distal tubular cells of the kidney. Isl-1 immunoreactivity was also widely distributed in neurons in both the central and peripheral nervous system, pituitary, and several human pituitary tumors and pheochromocytomas. The results of these studies implicate a potential role for isl-1 outside the endocrine pancreas in the nervous system and kidney.