Altered states:: Involvement of phosphorylated CagA in the induction of host cellular growth changes by Helicobacter pylori
Altered states:: Involvement of phosphorylated CagA in the induction of host cellular growth changes by Helicobacter pylori
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DOI：
10.1073/pnas.96.25.14559
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发表时间：
1999-12-07
影响因子：
11.1
通讯作者：
Tompkins, LS
中科院分区：
文献类型：
--
作者：
Segal, ED;Cha, J;Tompkins, LS
幽门螺杆菌存在于世界人口的一半，是一种非常成功的病原体。它可以在人体胃中存活数十年，对宿主几乎没有明显的影响。然而.它也是从胃炎到溃疡到胃癌的胃疾病的原因，并且已被世界卫生组织归类为1型致癌物。我们先前已经表明，145 kDa蛋白的磷酸化和信号转导通路的激活与H的附着有关。pylori到胃细胞。在这里，我们确定了145-kDa的蛋白质作为H。pylori CagA蛋白。我们还表明，CagA是必要的，以诱导生长因子样表型（蜂鸟）在宿主胃细胞类似的肝细胞生长因子（HGF）诱导。此外，我们确定了第二个细胞表型诱导后，附着H。pylori，我们称之为SFA（应激纤维相关）。SFA不依赖于CagA，由I型和II型H产生。幽门。
Helicobacter pylori, present in half of the world's population, is a very successful pathogen. It can survive for decades in the human stomach with few obvious consequences to the host. However. it is also the cause of gastric diseases ranging from gastritis to ulcers to gastric cancer and has been classified a type 1 carcinogen by the World Health Organization. We have previously shown that phosphorylation of a 145-kDa protein and activation of signal transduction pathways are associated with the attachment of H. pylori to gastric cells. Here we identify the 145-kDa protein as the H. pylori CagA protein. We also show that CagA is necessary to induce a growth-factor-like phenotype (hummingbird) in host gastric cells similar to that induced by hepatocyte growth factor (HGF). Additionally, we identify a second cellular phenotype induced after attachment by H. pylori, which we call SFA (stress fiber associated). SFA is CagA independent and is produced by type I and type II H. pylori.