Wnt/β-catenin signaling promotes regeneration after adult zebrafish spinal cord injury.
Wnt/β-catenin signaling promotes regeneration after adult zebrafish spinal cord injury.
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DOI：
10.1016/j.bbrc.2016.07.006
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发表时间：
2016-09
影响因子：
3.1
通讯作者：
Nicholas S. Strand;K. K. Hoi-K.;T. Phan;Catherine A. Ray;J. Berndt;R. Moon
中科院分区：
文献类型：
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作者：
Nicholas S. Strand;K. K. Hoi-K.;T. Phan;Catherine A. Ray;J. Berndt;R. Moon
与哺乳动物不同，斑马鱼可以再生受损的脊髓并重新控制尾部组织。最近的研究表明，Wnt/β-catenin信号在斑马鱼幼鱼脊髓再生中是必需的。然而，再生的分子机制可能会或可能不会被保存之间的幼虫和成年斑马鱼。为了验证这一点，我们评估了成年斑马鱼脊髓损伤后Wnt/β-catenin信号传导的作用。我们发现成年斑马鱼脊髓损伤后Wnt/β-catenin信号增加。此外，Dkk 1b的过表达抑制了成年斑马鱼再生脊髓中的Wnt/β-catenin信号传导。dkk 1b过表达也抑制运动恢复，轴突再生，神经胶质桥的形成在损伤的脊髓。因此，我们的数据说明了Wnt/β-catenin信号在成年和幼体斑马鱼脊髓再生中的保守作用。
Unlike mammals, zebrafish can regenerate their injured spinal cord and regain control of caudal tissues. It was recently shown that Wnt/β-catenin signaling is necessary for spinal cord regeneration in the larval zebrafish. However, the molecular mechanisms of regeneration may or may not be conserved between larval and adult zebrafish. To test this, we assessed the role of Wnt/β-catenin signaling after spinal cord injury in the adult zebrafish. We show that Wnt/β-catenin signaling is increased after spinal cord injury in the adult zebrafish. Moreover, overexpression of Dkk1b inhibited Wnt/β-catenin signaling in the regenerating spinal cord of adult zebrafish. Dkk1b overexpression also inhibited locomotor recovery, axon regeneration, and glial bridge formation in the injured spinal cord. Thus, our data illustrate a conserved role for Wnt/β-catenin signaling in adult and larval zebrafish spinal cord regeneration.