Multi-omics analysis provides insights into genetic architecture of flavonoid metabolites in Populus
Multi-omics analysis provides insights into genetic architecture of flavonoid metabolites in Populus
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多组学分析提供了对杨属类黄酮代谢物遗传结构的见解
DOI：
10.1016/j.indcrop.2021.113612
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发表时间：
2021
影响因子：
5.9
通讯作者：
Deqiang Zhang
中科院分区：
文献类型：
--
作者：
Wenjie Lu;Qingzhang Du;Liang Xiao;Chenfei Lv;Mingyang Quan;Peng Li;Liangchen Yao;Fangyuan Song;Deqiang Zhang
黄酮类化合物是一类广泛分布的次生代谢物，也是一类主要的化学防御物质，对光照、温度等外界因素做出反应。然而，对多年生树木中黄酮类化合物的遗传基础进行系统研究的文献很少。在本研究中，我们结合了基于代谢物的全基因组关联分析(MGwas)、表达数量性状基因座(EQTL)定位和加权共表达网络分析(WGCNA)来系统地构建杨中黄酮类化合物修饰的调控网络。首次对采自三个地理区域的300份毛白杨天然种质中的172种黄酮类化合物进行了特征分析。然后，我们进行了系统的遗传策略来筛选候选基因，构建了杨树类黄酮生物合成的调控网络。选择标记显示83种代谢物在杨树三个地理区域间表现出显著差异，因此我们在选择扫描分析的基础上确定了导致黄酮类化合物差异的候选基因。此外，我们还以控制花青素和二氢山奈酚水平的关键基因PtoLDOX为研究对象，通过等位基因频率和单倍型分析对其进行了分析。综上所述，我们的研究为揭示杨树类黄酮生物合成的遗传基础提供了新的见解，并为杨树不同地理区域的适应性选择提供了新的思路，为杨树品种的标记育种提供了可能。
Flavonoids are widely distributed secondary metabolites as well as a major class of chemical defenses in response to light intensity, temperature, and other external factors. However, systematic investigation on the genetic basis of flavonoids in perennial trees is poorly documented. In this study, we married metabolite-based genome wide association analysis (mGWAS), expression quantitative trait loci (eQTL) mapping and weighted co-expression network analysis (WGCNA) analysis to systematically construct the regulatory network underlying flavonoids decoration inPopulus. We firstly characterized 172 flavonoids features in 300 natural accessions ofPopulus tomentosacollected in three widespread geographical regions. Then, we conducted systematical genetic strategy to identify casual candidate genes, and constructed the regulatory network of flavonoids biosynthesis ofPopulus. Selection signature revealed 83 metabolites exhibited significant divergence among three geographical regions ofPopulus, we thus determined candidate genes contributing to flavonoids divergence based on the selection sweep analysis. Furthermore, we focused onPtoLDOX,a key gene responsible for the levels of anthocyanin and dihydrokaempferol, and dissected it through allelic frequency and haplotype analysis. Collectively, our study provides new insights into the genetic basis of flavonoids biosynthesis, and sheds light on the adaptive selection to different geographical regions that potentially dominated by the flavonoids ofPopulus, facilitating marker-based breeding in poplar varieties.