Controlled release hydrogen sulfide delivery system based on mesoporous silica nanoparticles protects graft endothelium from ischemia-reperfusion injury.
Controlled release hydrogen sulfide delivery system based on mesoporous silica nanoparticles protects graft endothelium from ischemia-reperfusion injury.
复制标题
基于介孔二氧化硅纳米粒子的控释硫化氢输送系统保护移植物内皮免受缺血再灌注损伤
DOI：
10.2147/ijn.s104604
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发表时间：
2016
影响因子：
8
通讯作者：
Wang C
中科院分区：
文献类型：
--
作者：
Wang W;Sun X;Zhang H;Yang C;Liu Y;Yang W;Guo C;Wang C
硫化氢在各种生理和病理过程中起着保护性气体传递的作用，但缺乏理想的供体严重阻碍了硫化氢的临床应用。本研究旨在构建一种可控释放的硫化氢供体，并评价其对移植血管内皮细胞的保护作用。采用溶胶凝胶法合成了介孔二氧化硅纳米颗粒(MSN)，并将其负载到硫化氢释放剂二烯丙基三硫化物(DATS)中。体外实验表明，DATS-MSN具有减轻内皮细胞炎症、促进内皮细胞增殖和迁移的作用。体内实验表明，DATS-MSN可减轻移植血管内皮细胞的凋亡。我们的结果表明，DATS-MSN以控制释放的方式释放H_2S，可以作为理想的H_2S供体。
Hydrogen sulfide (H2S) functions as a protective gas transmitter in various physiological and pathological processes, but the lack of ideal donors severely hampers the clinical application of H2S. This study aims to construct a controlled release H2S donor and evaluate its protective effect on graft endothelium. Mesoporous silica nanoparticles (MSNs) were synthesized using the sol–gel method and loaded with diallyl trisulfide (DATS), an H2S-releasing agent named DATS-MSN. In vitro experiments showed that DATS-MSN could alleviate endothelial cell inflammation and enhance endothelial cell proliferation and migration. In vivo experiments demonstrated that the apoptosis of graft endothelium was mitigated in the presence of DATS-MSN. Our results indicated that DATS-MSN, releasing H2S in a controlled release fashion, could serve as an ideal H2S donor.