Noninvasive photoacoustic and fluorescent tracking of optical dye labeled T cellular activities of diseased sites at new depth
Noninvasive photoacoustic and fluorescent tracking of optical dye labeled T cellular activities of diseased sites at new depth
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无创光声和荧光追踪新深度病变部位的光学染料标记的 T 细胞活动
DOI：
10.1002/jbio.201800073
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发表时间：
2018-09-01
影响因子：
2.8
通讯作者：
Nie，Liming
中科院分区：
文献类型：
--
作者：
Zheng，Shuai;Li，Honghui;Nie，Liming
免疫细胞的迁移对免疫反应至关重要。由于成像深度的原因，这些过程的可视化以前受到限制。我们开发了一种深穿透、灵敏和高分辨率的方法，使用快速光声断层扫描（PAT）在新的深度（高达9.5 mm）对淋巴结和疾病中T细胞的动态变化进行成像。将用NIR-797-异硫氰酸酯（一种优秀的近红外光声和荧光剂）标记的T细胞静脉注射到小鼠体内。我们使用荧光成像来粗略地确定T细胞的位置，并使用光声成像来深入细致地观察病变部位的T细胞反应。应用光声和荧光成像技术，无创观察淋巴结、急性疾病（细菌感染）和慢性疾病（肿瘤）中T细胞在不同时间点的动态变化。淋巴结和细菌感染部位的T细胞逐渐聚集，4h达高峰，随后下降。在肿瘤模型中，T细胞向肿瘤内迁移，在12小时达到最大值。本研究不仅为免疫活动的跟踪提供了一种新的观察方法，而且为治疗干预提供了连续监测。
The migration of immune cells is crucial to the immune response. Visualization of these processes has previously been limited because of the imaging depth. We developed a deep‐penetrating, sensitive and high‐resolution method to use fast photoacoustic tomography (PAT) to image the dynamic changes of T cells in lymph node and diseases at new depth (up to 9.5 mm). T cells labeled with NIR‐797‐isothiocyanate, an excellent near‐infrared photoacoustic and fluorescent agent, were intravenously injected to the mice. We used fluorescence imaging to determine the location of T cells roughly and photoacoustic imaging is used to observe T‐cell responses in diseased sites deeply and carefully. The dynamic changes of T cells in lymph node, acute disease (bacterial infection) and chronic disease (tumor) were observed noninvasively by photoacoustic and fluorescence imaging at different time points. T cells accumulated gradually and reached a maximum at 4 hours and declined afterwards in lymph node and bacterial infection site. At tumor model, T cells immigrated to the tumor with a maximum at 12 hours. Our study can not only provide a new observing method for immune activities tracking, but also enable continuous monitoring for therapeutic interventions.