TRANSFORMATION OF THEILERIA-PARVA DERIVED FROM AFRICAN BUFFALO (SYNCERUS-CAFFER) BY TICK PASSAGE IN CATTLE AND ITS USE IN INFECTION AND TREATMENT IMMUNIZATION
TRANSFORMATION OF THEILERIA-PARVA DERIVED FROM AFRICAN BUFFALO (SYNCERUS-CAFFER) BY TICK PASSAGE IN CATTLE AND ITS USE IN INFECTION AND TREATMENT IMMUNIZATION
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DOI：
10.1016/0304-4017(92)90043-9
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发表时间：
1992-06-01
影响因子：
2.6
通讯作者：
NGUMI, PN
中科院分区：
文献类型：
--
作者：
MARITIM, AC;YOUNG, AS;NGUMI, PN
用细小泰勒虫（Theileria parva）若虫饲养非洲布法罗（Syncerus caffer），获得稳定的小泰勒虫孢子（St.199），并通过感染和治疗方法免疫牛。90天后，将若虫蜱应用于其中一只阉牛，结果表明，所产生的成年蜱已被感染。采用蜱/牛传代方法，建立了T. Parva成立了。到第五次蜱/牛传代时，寄生虫种群的行为已经改变为T。来自牛的小孢子虫（parva）作为寄生虫在牛体内产生较高的双头寄生虫病和梨形虫病，并对蜱具有高度感染性。在急性感染期间，使用来自牛的感染细胞培养分离物对细小病毒进行检测。单克隆抗体谱显示，通过牛的过程中，寄生虫的抗原变化的证据很少，这是证实了在双向交叉免疫实验中使用子孢子稳定剂来自蜱从布法罗和牛的第四代。这些结果的意义，特别是在牛的免疫接种T。parva来源于布法罗。
A sporozoite stabilate (St. 199) of Theileria parva was obtained by feeding nymphal Rhipicephalus appendiculatus on an African buffalo (Syncerus caffer) and was used to immunize cattle by the infection and treatment method. Nymphal ticks were applied to one of the steers 90 days later and it was shown that the resultant adult tick had become infected. Using tick/cattle passage, two passage lines of T. parva were established. By the fifth tick/cattle passage, the parasite stocks had changed their behaviour to that of T. parva derived from cattle as the parasite produced relatively high schizont parasitosis and piroplasm parasitaemia in cattle, and had become highly infective to ticks.At various passage levels the parasite populations were characterized by behaviour and by monoclonal antibodies against T. parva schizonts using infected cell culture isolates from cattle during acute infections. The monoclonal antibody profile showed little evidence of antigen change of the parasite during passage through cattle, which was confirmed in a two-way cross-immunity experiment using sporozoite stabilate derived from ticks obtained from the buffalo and fourth passage in cattle. The implication of these results, particularly in relationship to immunization of cattle against T. parva derived from buffalo, is discussed.