MORTALITY OF LEISHMANIA-MAJOR IN PHLEBOTOMUS-PAPATASI CAUSED BY PLANT-FEEDING OF THE SAND FLIES
MORTALITY OF LEISHMANIA-MAJOR IN PHLEBOTOMUS-PAPATASI CAUSED BY PLANT-FEEDING OF THE SAND FLIES
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DOI：
10.4269/ajtmh.1994.50.1.tm0500010020
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发表时间：
1994-01-01
影响因子：
3.3
通讯作者：
JACOBSON, RL
中科院分区：
文献类型：
--
作者：
SCHLEIN, Y;JACOBSON, RL
本文研究了帕帕塔斯白蛉的植物性摄食及植物性饵料对感染利什曼原虫的影响。以植物为食的果蝇在其肠道中有小的游离颗粒和膜碎片，这些颗粒和碎片被calcofluor染色为纤维素植物组织。取食刺山柑后，在34.0%的雌性和14.3%的雄性白蛉体内发现了这种蛋白质。没有发现植物残留物，在54名女性已被喂食植物来源的蜜露分泌物的蚜虫craccivora提供的分支的主机。未进食果蝇的肠腔中也没有Calcofluor-stained颗粒。无论植物组织的摄入量如何，在提供了刺山柑植物或蜜露的系列中，糖喂养的比例通过测试肠道中果糖的存在来估计。果糖阳性果蝇的比例在每个系列中，男性和女性，是45%。通过在约旦河谷的32.8%的雌性和10.3%的雄性帕帕塔斯的肠道中发现组织残留物，证明了田间植物取食。利用充满食用染料的树枝诱饵，并在野生捕获的P. papatasi中找到染料标记，证明了对特定植物的摄食。研究了植物性饲料对L.主要的感染如下：锦葵和冰蜡属的蜜露产生大量的寄生虫血症;然而，取食蓖麻、刺山柑和黄茄分别在88%、55%和46%的感染中引起> 50%的寄生虫死亡和变形。这种类型的损伤也观察到在21的38个成熟的感染现场捕获的苍蝇。这些观察结果表明，一些植物饲料的野生木瓜，损害L。主要感染苍蝇，从而降低其传播利什曼病的能力。
The plant feeding of Phlebotomus papatasi and the effects of plant diets on the Leishmania major infections were investigated. Plant-fed flies had small free particles and membranous shreds in their gut that were stained by calcofluor as cellulosic plant tissues. They were found in 34.0% of the female and 14.3% of the male sand flies following feeding on the caper plant (Capparis spinosa). No plant residues were found in 54 females that had been fed on plant-derived honeydew secretions of Aphis craccivora offered on a branch of the host plant. Calcofluor-stained particles were also absent from the gut lumen of unfed flies. The proportion of sugar feeding, regardless of the intake of plant tissue, in the series that had been offered caper plant or honeydew was estimated by testing for the presence of fructose in the gut. The proportion of fructose-positive flies in each series, among both males and females, was 45%. Plant feeding in the field was demonstrated by finding tissue residues in the gut of 32.8% of female and 10.3% of male P. papatasi from the Jordan Valley. Feeding on specific plants was demonstrated using baits of branches suffused with food dye and finding the dye marker in wild-caught P. papatasi. The influence of plant diets on L. major infections in P. papatasi was as follows: Malva nicaeensis and the honeydew of Icerya purchasi produced thriving parasitemias; however, feeding on Ricinus communis, Capparis spinosa, and Solanum luteum caused > 50% mortality and deformation of parasites in 88%, 55%, and 46% of the infections, respectively. This type of injury was also observed in 21 of the 38 mature infections in field-caught flies. These observations imply that some plant diets of P. papatasi in the wild impair L. major infections in the flies, thereby decreasing their capacity to transmit leishmaniasis.