Peripheral blood CD14+ cells from healthy subjects carry a circular conformation of latent cytomegalovirus genome
Peripheral blood CD14+ cells from healthy subjects carry a circular conformation of latent cytomegalovirus genome
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DOI：
10.1182/blood.v93.1.394.401k44_394_398
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发表时间：
1999-01-01
期刊：
影响因子：
20.3
通讯作者：
Wiedeman, JA
中科院分区：
文献类型：
--
作者：
Bolovan-Fritts, CA;Mocarski, ES;Wiedeman, JA
大多数人群都携带潜伏的巨细胞病毒。尽管CD14(+)外周血单核细胞被认为是潜伏位点，但这些细胞中潜伏病毒基因组的构象尚不清楚。在这项研究中，使用天然琼脂糖凝胶电泳分离结合聚合酶链式反应检测，评估了来自健康病毒血清阳性携带者的 CD14+ 细胞中病毒基因组 DNA 的构象。在这里，我们展示了病毒基因组作为环形质粒迁移，其迁移率相当于环形 230-kb 福氏志贺氏菌巨质粒标记。没有检测到病毒基因组的线性形式、复杂形式或整合形式。该报告进一步证明CD14(+)细胞群是自然感染人类宿主病毒潜伏的重要部位。在潜伏期间检测到病毒基因组为环状质粒，表明该病毒以类似于其他疱疹病毒的方式维持其基因组，其中潜伏病毒基因组构象已被研究。 (C) 1999 年，美国血液学会。
The majority of the human population harbors latent cytomegalovirus. Although CD14(+) peripheral blood mononuclear cells have been implicated as sites of latency, the conformation of the latent viral genome in these cells is unknown. In this study, the conformation of viral genomic DNA was assessed in CD14+ cells from healthy virus seropositive carriers using an electrophoretic separation on native agarose gels in combination with polymerase chain reaction detection. Here we show that the viral genome migrates as a circular plasmid with a mobility equivalent to a circular 230-kb Shigella flexneri megaplasmid marker. Neither linear nor complex or integrated forms of the viral genome were detected. This report provides further evidence that the CD14(+) cell population is an important site of viral latency in the naturally infected human host. Detection of the viral genome as a circular plasmid during latency suggests that this virus maintains its genome in a manner analogous to other herpesviruses where latent viral genome conformation has been studied. (C) 1999 by The American Society of Hematology.