The effects of nanoparticles on mouse testis Leydig cells in vitro
The effects of nanoparticles on mouse testis Leydig cells in vitro
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DOI：
10.1016/j.tiv.2008.08.009
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发表时间：
2008-12-01
影响因子：
3.2
通讯作者：
Takeda, Ken
中科院分区：
文献类型：
--
作者：
Komatsu, Tomoko;Tabata, Masako;Takeda, Ken
我们已经指出，柴油废气颗粒(DEP)和二氧化钛(Ti02)等纳米颗粒可能会损害雄性小鼠的生殖系统。在这项研究中，为了评估纳米颗粒对睾丸组成细胞的直接影响，我们检测了DEP、二氧化钛和碳黑(CB)对小鼠睾丸间质TM3细胞(睾丸产生睾酮的细胞)的影响。用透射电子显微镜或场发射型扫描电子显微镜/能量色散X-射线能谱(FE-SEM/EDS)检测三种纳米粒子在间质细胞中的摄取情况。我们检测了纳米粒子的细胞毒性和对基因表达的影响。二氧化钛对间质细胞的细胞毒作用强于其他纳米粒子。在氧化应激敏感标志物--血红素氧合酶-1(HO-1)的表达被DER显著诱导的同时，CB和DEP使控制线粒体胆固醇转运的类固醇合成急性调节蛋白(STAR)的基因表达略有增加。在本研究中，我们发现DEPS、二氧化钛和CB纳米颗粒会被间质细胞摄取，并影响细胞的存活、增殖和基因表达。每个纳米颗粒的图案都是独一无二的。(C)2008年，爱思唯尔有限公司出版。
We have indicated the possibility that nanoparticles such as diesel exhaust particles (DEP) and titanium dioxide (TiO2) may impair the male Mouse reproductive system. In this study, to evaluate the direct effect of nanoparticles on testis-constituent cells, we examined the effect of DEP, TiO2 and carbon black (CB) on mouse Leydig TM3 cells, the testosterone-producing cells of the testis. The uptake of three nanoparticles into Leydig cells was detected using transmission electron microscopy (TEM) or field emission type scanning electron microscopy/energy-dispersive X-ray spectroscopy (FE-SEM/EDS). We examined the cytotoxicity and the effect on gene expression by treatment with narroparticles. TiO2 was more cytotoxic to Leydig cells than other narroparticles. The proliferation of Leydig cells was suppressed transiently by treatment with TiO2 or DER The expression of heme oxygenase-1 (HO-1), a sensitive marker for oxidative stress, was induced remarkably by treatment with DER Furthermore, CB and DEP slightly increased the gene expression of the steroidogenic acute regulatory (StAR) protein, the factor that controls mitochondrial cholesterol transfer. In this study, we found that DEPs, TiO2 and CB nanoparticles were taken up by Leydig cells, and affected the viability, proliferation and gene expression. The patterns were unique for each nanoparticle. (C) 2008 Published by Elsevier Ltd.