Regulation of tryptophan hydroxylase by cyclic AMP, calcium, norepinephrine, and light in cultured chick pineal cells.
Regulation of tryptophan hydroxylase by cyclic AMP, calcium, norepinephrine, and light in cultured chick pineal cells.
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培养鸡松果体细胞中环磷酸腺苷、钙、去甲肾上腺素和光对色氨酸羟化酶的调节。
DOI：
10.1046/j.1471-4159.1996.67010242.x
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发表时间：
1996
影响因子：
4.7
通讯作者：
Takahashi,JS
中科院分区：
文献类型：
--
作者：
Florez,JC;Takahashi,JS
培养的鸡松果体细胞色氨酸羟化酶（TPH）的35S参入水平呈昼夜节律。TPH振荡在主观夜晚早期达到峰值，在恒定的黑暗中持续存在，并且可以通过光进行相移，与这些治疗对褪黑激素合成的影响平行。使用定量二维聚丙烯酰胺凝胶电泳，我们已经研究了已知影响鸡松果体褪黑激素合成的药物对TPH的调节。我们在这里报告，35S掺入TPH诱导的cAMP和钙，并部分抑制急性曝光。环磷酸腺苷还导致TPH亚型之一的放射性标记成比例增加，同时另一种亚型减少，可能反映了TPH磷酸化状态的变化。这种作用是逆转的治疗已知降低细胞内环磷酸腺苷水平在鸡松果体。因此，环磷酸腺苷似乎参与了翻译和翻译后过程调节表达的TPH在鸡松果体细胞。
The level of35S incorporation into tryptophan hydroxylase (TPH) shows a circadian rhythm in cultured chick pineal cells. The TPH oscillation peaks in the early subjective night, persists in constant darkness, and can be phase shifted by light, in parallel to the effect of these treatments on melatonin synthesis. Using quantitative two‐dimensional polyacrylamide gel electrophoresis, we have examined the regulation of TPH by agents known to affect melatonin synthesis in the chick pineal. We report here that35S incorporation into TPH is induced by cyclic AMP and calcium, and partially inhibited by acute exposure to light. Cyclic AMP also causes a proportional increase in the radiolabeling of one of the TPH isoforms and a concomitant decrease in another isoform, possibly reflecting a change in the phosphorylation state of TPH. This effect is reversed by treatments known to reduce intracellular cyclic AMP levels in the chick pineal. Cyclic AMP thus appears to be involved in both translational and posttranslational processes regulating the expression of TPH in chick pineal cells.