Comparison of bacterial carbon production estimates from dilution and 3H ‐leucine methods across a strong gradient in ocean productivity
Comparison of bacterial carbon production estimates from dilution and 3H ‐leucine methods across a strong gradient in ocean productivity
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在海洋生产力的强梯度上比较稀释法和 3H - 亮氨酸方法的细菌碳产量估计值
DOI：
10.1002/lom3.10546
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发表时间：
2023
期刊：
影响因子：
--
通讯作者：
Selph, Karen E.
中科院分区：
文献类型：
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作者：
Landry, Michael R.;Rivera, Sara R.;Stukel, Michael R.;Selph, Karen E.
3h标记亮氨酸进入蛋白质的吸收是一种广泛使用的估计细菌碳产量（BCP）的方法，被认为低估或高估了开放海洋中细菌的生长，其不确定性系数为40。与此同时，另一种BCP方法，即稀释法，对过滤释放的溶解底物的细菌生长的潜在高估存在未经验证的担忧。我们比较了加州洋流生态系统中从近海少营养化到沿海上升流的三次巡航的BCPDiland bcpleu估计。我们的初步分析基于正午细菌大小的显微镜估计和转换关系的先验假设，结果显示BCP估计（BCPDilhigher）平均相差两倍，但在低生产力和高生产力工作站之间没有系统偏差。BCPDiland bcpleu均与细菌细胞丰度密切相关。重新分析结果，涉及不同的细胞碳-生物体积关系，并通过流式细胞术的前角光散射作为相对细胞大小指数，证明BCPDiland bcpleube完全兼容，与5 fg C细胞−1的细菌1:1适合。基于这些结果并考虑到方法的不同优势，3h标记亮氨酸和稀释技术的联合使用对细菌生物量和产量提供了强有力的相互支持的约束。
The uptake of3H‐labeled leucine into proteins, a widely used method for estimating bacterial carbon production (BCP), is suggested to underestimate or overestimate bacterial growth in the open ocean by a factor of 40 uncertainty. Meanwhile, an alternative BCP approach, by the dilution method, has untested concerns about potential overestimation of bacterial growth from dissolved substrates released by filtration. We compared BCPDiland BCPLeuestimates from three cruises across a broad trophic gradient, from offshore oligotrophy to coastal upwelling, in the California Current Ecosystem. Our initial analyses based on midday microscopical estimates of bacterial size and a priori assumptions of conversions relationships revealed a mean two‐fold difference in BCP estimates (BCPDilhigher), but no systematic bias between low and high productivity stations. BCPDiland BCPLeuboth demonstrated strong relationships with bacteria cell abundance. Reanalysis of results, involving a different cell carbon‐biovolume relationship and informed by forward angle light scatter from flow cytometry as a relative cell size index, demonstrated that BCPDiland BCPLeuare fully compatible, with a 1 : 1 fit for bacteria of 5 fg C cell−1. Based on these results and considering different strengths of the methods, the combined use of3H‐labeled leucine and dilution techniques provide strong mutually supportive constraints on bacterial biomass and production.