Severe developmental defects, hypersensitivity to DNA-damaging agents, and lengthened telomeres in Arabidopsis MRE11 mutants
Severe developmental defects, hypersensitivity to DNA-damaging agents, and lengthened telomeres in Arabidopsis MRE11 mutants
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DOI：
10.1105/tpc.005959
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发表时间：
2002-10-01
期刊：
影响因子：
11.6
通讯作者：
Hooykaas, P
中科院分区：
文献类型：
--
作者：
Bundock, P;Hooykaas, P
Mre11蛋白对细胞的长期遗传稳定性至关重要，并确保DNA损伤的有效修复。缺乏Mre11功能的脊椎动物细胞不能存活。然而，我们在这里报道，在模式植物拟南芥中情况并非如此。我们分离了两种不同的拟南芥系，其中含有整合在MRE11基因不同点上的T-DNA拷贝（AtMRE11）。两种突变株都对dna损伤处理敏感，但表现出明显不同的发育表型。此外，我们还在这些植物品系中观察到端粒延长，表明AtMre11参与端粒维持。因此，我们分离的细胞系是详细研究AtMre11在成熟植物中的作用的独特工具。
The Mre11 protein is essential for the long-term genetic stability of the cell and acts to ensure the efficient repair of DNA damage. Vertebrate cells lacking Mre11 function are not viable. However, we report here that this is not the case in the model plant Arabidopsis. We have isolated two different Arabidopsis lines containing a T-DNA copy integrated at a different point in the MRE11 gene (AtMRE11). Both mutant plant lines were hypersensitive to DNA-damaging treatments but exhibited strikingly different developmental phenotypes. Furthermore, we also observed lengthened telomeres in these plant lines, showing that AtMre11 is involved in telomere maintenance. Thus, the lines we have isolated are unique tools with which to study in detail the role of AtMre11 in the mature plant.