The effect of alkaline borohydride treatment on N-linked carbohydrates of glycoproteins.
The effect of alkaline borohydride treatment on N-linked carbohydrates of glycoproteins.
复制标题
碱性硼氢化物处理对糖蛋白的 N-连接碳水化合物的影响。
DOI：
10.1007/bf01047889
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发表时间：
1989
影响因子：
3
通讯作者：
Alhadeff,JA
中科院分区：
文献类型：
--
作者：
Argade,SP;DavesJr,GD;VanHalbeek,H;Alhadeff,JA
本文系统地研究了碱性硼氢化钠和硼氢化钠水溶液对糖蛋白核糖核酸酶-B、蛋白质核糖核酸酶-A和肌红蛋白以及糖基氨基酸GlcNAcβ（1-N）Asn的处理效果与试剂浓度、温度和处理时间的关系。使用高场1H-NMR光谱、色谱法和氨基酸分析来表征各种化合物的处理的产物。我们的研究结果表明，温和的碱性硼氢化物处理，以及单独的硼氢化物水溶液处理，是能够广泛降解多肽和部分释放的N-连接的聚糖从核糖核酸酶-B。最初，产生糖肽，其肽部分由几种氨基酸组成，其进一步水解以产生比例为104：1的糖-天冬酰胺和寡糖-糖醇的混合物。强碱性硼氢化物处理核糖核酸酶-B能够完全释放N-连接的碳水化合物作为寡糖-糖醇。
The effects of treatments of the glycoprotein ribonuclease-B, the proteins ribonuclease-A and myoglobin, and the glyco-amino acid GlcNAcβ(1-N) Asn with alkalil alkaline sodium borohydride, and aqueous sodium borohydride were systematically studied as a function of the concentration of the reagents, the temperature, and the length of the treatment. High-field1H-NMR spectroscopy, chromatographic methods and amino-acid analysis were used to characterize products of the treatments of the various compounds. Our results indicate that mild alkaline borohydride treatment, as well as aqueous borohydride treatment alone, is capable of extensively degrading polypeptides and of partially releasing theN-linked glycans from ribonuclease-B. Initially, glycopeptides are produced, the peptide portion of which consists of several amino acids, which are further hydrolyzed to yield a mixture of glyco-asparagines and oligosaccharide-alditols in the ratio of ≈4:1. Strong alkaline borohydride treatment of ribonuclease-B is capable of completely releasing theN-linked carbohydrates as oligosaccharide-alditols.