Direct interaction of aminopeptidase A with recombination site DNA in Xer site-specific recombination
Direct interaction of aminopeptidase A with recombination site DNA in Xer site-specific recombination
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DOI：
10.1093/emboj/16.17.5188
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发表时间：
1997-09-01
期刊：
影响因子：
11.4
通讯作者：
Colloms, SD
中科院分区：
文献类型：
--
作者：
Alen, C;Sherratt, DJ;Colloms, SD
Xer在ColE1 cer处的定点重组将质粒多聚体转化为单体，从而保证了ColE1的遗传稳定性。两个相关的重组酶蛋白XerC和XerD在30 bp重组核心位点催化链交换反应，此外，cer处的重组还需要两个辅助蛋白PepA和ArgR。这两种辅助蛋白被认为在与 cer 重组核心相邻的 180 bp 辅助序列上起作用，以确保重组仅发生在同一分子上的直接重复位点之间。在这里，我们证明 PepA 和 ArgR 直接与 cer 相互作用，形成一个复合物，其中两个 cer 位点的辅助序列以右手方式缠绕大约 3 次。我们提出了这种突触复合物的模型，并提出链交换只能在该复合物形成后发生。
Xer site-specific recombination at ColE1 cer converts plasmid multimers into monomers, thus ensuring the heritable stability of ColE1, Two related recombinase proteins, XerC and XerD, catalyse the strand exchange reaction at a 30 bp recombination core site, In addition, two accessory proteins, PepA and ArgR, are required for recombination at cer. These two accessory proteins are thought to act at 180 bp of accessory sequences adjacent to the cer recombination core to ensure that recombination only occurs between directly repeated sites on the same molecule, Here, we demonstrate that PepA and ArgR interact directly with cer, forming a complex in which the accessory sequences of two cer sites are interwrapped approximately three times in a right-handed fashion, We present a model for this synaptic complex, and propose that strand exchange can only occur after the formation of this complex.