Identification of intelectin overexpression in malignant pleural mesothelioma by serial analysis of gene expression (SAGE)
Identification of intelectin overexpression in malignant pleural mesothelioma by serial analysis of gene expression (SAGE)
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DOI：
10.1016/j.lungcan.2004.10.011
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发表时间：
2005-04-01
期刊：
影响因子：
5.3
通讯作者：
Pass, HI
中科院分区：
文献类型：
--
作者：
Wali, A;Morin, PJ;Pass, HI
恶性胸膜间皮瘤（MPM）是一种致命的肿瘤，没有可接受的治疗方法。我们使用基因表达序列分析（SAGE）来比较手术切除的MPM与自体正常间皮的基因表达模式。在肿瘤中发现了智力素基因过表达（> - 139倍）。在线SAGE数据集显示，在间皮瘤、卵巢癌和结肠癌中一致存在智力素。RT-PCR检测5例切除的MPM肿瘤中有4例表达了inelectin mRNA，免疫组化检测证实53例MPM肿瘤中有28例表达了inelectin蛋白，Western blot检测4例间皮瘤细胞中有4例表达了inelectin蛋白。由于石棉石棉暴露和猿猴病毒40感染，在人原代间皮细胞中观察到明显的智力素基因表达诱导。间皮瘤中白细胞介素的过表达可能具有潜在的筛查和治疗意义。2004爱思唯尔爱尔兰有限公司版权所有。
Malignant pleural mesothelioma (MPM) is a fatal neoplasm with no acceptable curative approaches. We used serial analysis of gene expression (SAGE) to compare the gene expression pattern of a surgically resected MPM to the autologous normal mesothelium. Intelectin gene overexpression (> 139-fold) was found in the tumor. Online SAGE datasets revealed intelectin to be consistently present in mesothelioma(s), ovarian cancer, and colon cancer. Intelectin mRNA expression was found by RT-PCR in 4 of 5 resected MPM tumors, and Intelectin protein expression was confirmed by immunohistochemistry in 28 of 53 MPM tumors, and in 4 of 4 mesothelioma cell tines studied by Western blot. A marked induction in intelectin gene expression was observed among human primary mesothelial cells as a consequence of crocidolite asbestos exposure and simian virus 40 infection. Intelectin overexpression in mesothelioma could have potential screening, and therapeutic implications. (c) 2004 Elsevier Ireland Ltd. All rights reserved.