Derepression of ethylene-stabilized transcription factors (EIN3/EIL1) mediates jasmonate and ethylene signaling synergy in Arabidopsis
Derepression of ethylene-stabilized transcription factors (EIN3/EIL1) mediates jasmonate and ethylene signaling synergy in Arabidopsis
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拟南芥中乙烯稳定转录因子 (EIN3/EIL1) 的去阻遏介导茉莉酸和乙烯信号协同作用
DOI：
10.1073/pnas.1103959108
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发表时间：
2011-07-26
影响因子：
11.1
通讯作者：
Guo, Hongwei
中科院分区：
文献类型：
--
作者：
Zhu, Ziqiang;An, Fengying;Guo, Hongwei
茉莉酸（JA）和乙烯（ET）是两种主要的植物激素，协同调节植物的发育和对坏死营养型真菌的耐受性。JA和ET都诱导几个发病机制相关基因的表达，而阻断任何一个信号转导途径都可以取消JA和ET单独或联合对这些基因的诱导。然而，JA/ET相互作用和信号相互依赖的分子基础在很大程度上是未知的。在这里，我们报告，两个拟南芥ET稳定的转录因子（EIN 3和EIL 1）整合ET和JA信号在基因表达，根发育和necrotrophic病原体防御的调节。进一步的研究表明，JA通过去除JA-Zim结构域（JAZ）蛋白增强EIN 3/EIL 1的转录活性，JAZ蛋白与EIN 3/EIL 1物理相互作用并抑制EIN 3/EIL 1。此外，我们发现JAZ蛋白招募RPD 3型组蛋白脱乙酰酶（HDA 6）作为辅助阻遏物，调节组蛋白乙酰化，抑制EIN 3/EIL 1依赖性转录，并抑制JA信号。我们的研究确定EIN 3/EIL 1作为一个关键的整合节点，其激活需要JA和ET信号，并说明转录去抑制作为一个共同的机制，以整合不同的信号通路在植物发育和防御的调节。
Jasmonate (JA) and ethylene (ET) are two major plant hormones that synergistically regulate plant development and tolerance to necrotrophic fungi. Both JA and ET induce the expression of several pathogenesis-related genes, while blocking either signaling pathway abolishes the induction of these genes by JA and ET alone or in combination. However, the molecular basis of JA/ET coaction and signaling interdependency is largely unknown. Here, we report that two Arabidopsis ET-stabilized transcription factors (EIN3 and EIL1) integrate ET and JA signaling in the regulation of gene expression, root development, and necrotrophic pathogen defense. Further studies reveal that JA enhances the transcriptional activity of EIN3/EIL1 by removal of JA-Zim domain (JAZ) proteins, which physically interact with and repress EIN3/EIL1. In addition, we find that JAZ proteins recruit an RPD3-type histone deacetylase (HDA6) as a corepressor that modulates histone acetylation, represses EIN3/EIL1-dependent transcription, and inhibits JA signaling. Our studies identify EIN3/EIL1 as a key integration node whose activation requires both JA and ET signaling, and illustrate transcriptional derepression as a common mechanism to integrate diverse signaling pathways in the regulation of plant development and defense.