Caveolae-mediated Endocytosis of Intratracheally Instilled Gold Colloid Nanoparticles at the Air-Blood Barrier in Mice
Caveolae-mediated Endocytosis of Intratracheally Instilled Gold Colloid Nanoparticles at the Air-Blood Barrier in Mice
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DOI：
10.1177/0192623312457271
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发表时间：
2013-04-01
影响因子：
1.5
通讯作者：
Takano, Hirohisa
中科院分区：
文献类型：
--
作者：
Naota, Misaki;Shimada, Akinori;Takano, Hirohisa
内吞作用是纳米颗粒在肺泡上皮上移位的主要机制。本研究的目的是阐明内吞作用和纳米颗粒在空气-血液屏障（ABB）的移位之间的关联。将金胶体颗粒（直径，20 nm）经气管内滴入雄性ICR小鼠中。滴注后15分钟，在巨噬细胞的细胞质中、肺泡上皮细胞（AEC）的表面上和肺泡中观察到聚集的金颗粒的局部积聚。电子显微镜下发现颗粒的巨噬细胞的囊泡，表面上的AEC，并在小窝样囊泡在1型AEC。免疫组织化学显示未处理的肺以及用金颗粒处理的肺的ABB中的小窝蛋白-1的免疫标记阳性。双免疫荧光和免疫电子显微镜显示存在的小窝蛋白-1在AEC在未经处理的肺。这些结果表明，滴注的金胶体颗粒通过小窝介导的内吞作用内化到ABB的肺泡上皮中，这被认为是AEC的生理功能。
Endocytosis is the primary mechanism by which nanoparticles are translocated over the alveolar epithelium. The purpose of this study was to elucidate the association between endocytosis and the translocation of nanoparticles at the air-blood barrier (ABB). Gold colloid particles (diameter, 20 nm) were intratracheally instilled into male ICR mice. Fifteen minutes after instillation, localized accumulation of agglomerated gold particles was observed in the cytoplasm of macrophages, on the surface of alveolar epithelial cells (AECs), and in alveoli. Electron microscopy revealed particles in the vesicles of macrophages, on the surface of AECs, and in caveolae-like vesicles in type 1 AECs. Immunohistochemistry demonstrated positive immunolabeling for caveolin-1 in the ABB of untreated lungs as well as lungs treated with gold particles. Double immunofluorescence and immunoelectron microscopy revealed the presence of caveolin-1 in AECs in the untreated lungs. These results suggest that instilled gold colloid particles are internalized into the alveolar epithelium at the ABB by caveolae-mediated endocytosis, which is regarded as a physiological function of AECs.