Eutopic endometrium from women with endometriosis does not exhibit neurotrophic properties
Eutopic endometrium from women with endometriosis does not exhibit neurotrophic properties
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DOI：
10.1016/j.jneuroim.2012.04.020
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发表时间：
2012-08-15
影响因子：
3.3
通讯作者：
Mechsner, Sylvia
中科院分区：
文献类型：
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作者：
de Arellano, Maria Luisa Barcena;Arnold, Julia;Mechsner, Sylvia
采用免疫荧光染色、蛋白质印迹和神经元生长试验，对子宫内膜异位症患者在位子宫内膜中神经营养因子的作用进行了前瞻性研究。神经生长因子在患有和不患有子宫内膜异位症的女性的原代子宫内膜细胞培养物中表达。对子宫内膜异位症患者和非子宫内膜异位症患者的子宫内膜活检或子宫液进行蛋白质印迹分析，结果显示神经营养因子的表达无差异。我们没有发现子宫内膜异位症患者和非子宫内膜异位症患者在接受条件培养液或子宫液治疗时感觉神经节突起生长方面的差异。这一结果驳斥了子宫内膜异位症患者在位子宫内膜具有神经营养特性的假设。(C)2012爱思唯尔有限公司版权所有。
The role of neurotrophins in eutopic endometrium from endometriosis-patients was investigated in a prospective study using immunofluorescence-staining, Western blot and a neuronal growth assay. The nerve growth factor is expressed in primary endometrial cell culture from women with and without endometriosis. Western blot analysis of endometrial biopsies or uterine fluid from patients with and without endometriosis shows no difference in the neurotrophin expression. We could not find a difference between patients with and without endometriosis with regards to the neurite outgrowth of sensory ganglia when treated with conditioned cultured medium or uterine fluid. This result refutes the assumed neurotrophic properties of eutopic endometrium of patients with endometriosis. (C) 2012 Elsevier B.V. All rights reserved.