Gamma aminobutyric acid immunoreactivity in the mouse adrenal gland during postnatal development.
Gamma aminobutyric acid immunoreactivity in the mouse adrenal gland during postnatal development.
复制标题
出生后发育过程中小鼠肾上腺的γ-氨基丁酸免疫反应性。
DOI：
10.1679/aohc.61.373
复制
发表时间：
1998
影响因子：
--
通讯作者：
H. Tanaka
中科院分区：
文献类型：
--
作者：
K. Iwasa;Y. Oomori;H. Tanaka
为了了解小鼠肾上腺中含γ-氨基丁酸（GABA）的嗜铬细胞和神经纤维的发育过程，我们用GABA抗体对出生后不同阶段的组织进行了免疫组织化学检查。从出生到出生后第1周，GABA免疫反应被认为是在很少的神经纤维，并在没有嗜铬细胞。在出生后第2周，GABA免疫反应性出现在集群的嗜铬细胞和强烈的相对众多的静脉曲张的神经纤维。免疫反应阳性神经纤维密集分布于免疫反应阴性的小嗜铬细胞和大神经节细胞，而仅稀疏分布于免疫反应弱的嗜铬细胞。在生后3周，免疫反应阳性的嗜铬细胞和神经纤维的数量进一步增加，与生后2周相比。GABA免疫反应阳性神经纤维在髓质的染色模式是相似的，在出生后第2周。从出生后4周到出生后8周，免疫反应阳性的嗜铬细胞和神经纤维的分布和频率也与出生后3周相似。这些结果表明，GABA在小鼠肾上腺的嗜铬细胞和神经纤维的表达可能是在出生后3周完成。
To understand the developing processes of gamma-aminobutyric acid (GABA)-containing chromaffin cells and nerve fibers in the mouse adrenal gland, we examined the tissues in various postnatal stages by immunohistochemistry using a GABA antibody. From birth until postnatal week 1, GABA-immunoreactivity was seen in very few nerve fibers, and in none of the chromaffin cells. At postnatal week 2, GABA-immunoreactivity appeared weakly in clusters of chromaffin cells and strongly in relatively numerous varicose nerve fibers. The immunoreactive nerve fibers were densely distributed in the small immunonegative chromaffin cells and large ganglion cells, but only sparsely so in the weak immunoreactive chromaffin cells. At postnatal week 3, the number of the immunoreactive chromaffin cells and nerve fibers further increased compared to that at postnatal week 2. The staining pattern of GABA-immunoreactive nerve fibers in the medullas was similar to that at postnatal week 2. From postnatal week 4 until postnatal week 8, the distribution and frequency of the immunoreactive chromaffin cells and nerve fibers were also similar to those at postnatal week 3. These results suggest that the expression of GABA in the chromaffin cells and in the nerve fibers of the mouse adrenal gland may be completed by postnatal week 3.