Comparison of the effects of e-cigarette vapor with cigarette smoke on lung function and inflammation in mice
Comparison of the effects of e-cigarette vapor with cigarette smoke on lung function and inflammation in mice
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DOI：
10.1152/ajplung.00389.2017
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发表时间：
2018-11-01
影响因子：
4.9
通讯作者：
Papapetropoulos, Andreas
中科院分区：
文献类型：
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作者：
Glynos, Constantinos;Bibli, Sofia-Iris;Papapetropoulos, Andreas
电子烟（e-cigs）被宣传为一种危害较小的尼古丁输送系统或一种新的戒烟工具。我们的目的是评估电子烟蒸汽在肺部的体内影响，并将其与香烟烟雾（CS）进行比较。我们将C57 BL/6小鼠暴露于环境空气、CS或电子烟蒸汽3天或4周，并测定氧化应激、炎症和肺力学，所述环境空气、CS或电子烟蒸汽含有1）丙二醇/植物甘油（PG：VG-Sol; 1：1）、2）PG：VG与尼古丁（G：VG-N）或3）PG：VG与尼古丁和香料（PG：VG-N + F）。电子烟蒸汽，特别是PG：VG-N + F，增加支气管肺泡灌洗液（BALF）细胞结构，Muc 5ac产生，以及BALF和肺氧化应激标志物至少100%，在许多情况下超过CS。研究的两个时间点的BALF蛋白含量仅在PG：VG-N + F组中升高。3天后，PG：VG-Sol改变了组织弹性、静态顺应性和气道阻力，而4周后CS是唯一对这些参数产生不利影响的治疗。CS和PG：VG-N + F组对乙酰甲胆碱的气道高反应性增加相似。我们的研究结果表明，暴露于电子烟蒸汽可以引发炎症反应，并对呼吸系统力学产生不利影响。在许多情况下，电子烟中添加的香料加剧了电子烟蒸汽的有害影响。我们的结论是，电子烟和传统香烟吸烟都对肺部生物学产生负面影响。
Electronic cigarettes (e-cigs) are advertised as a less harmful nicotine delivery system or as a new smoking cessation tool. We aimed to assess the in vivo effects of e-cig vapor in the lung and to compare them to those of cigarette smoke (CS). We exposed C57BL/6 mice for either 3 days or 4 wk to ambient air, CS, or e-cig vapor containing 1) propylene glycol/vegetable glycerol (PG: VG-Sol; 1:1), 2) PG:VG with nicotine (G:VG-N), or 3) PG:VG with nicotine and flavor (PG:VG-N + F) and determined oxidative stress, inflammation, and pulmonary mechanics. E-cig vapors, especially PG:VG-N + F, increased bronchoalveolar lavage fluid (BALF) cellularity, Muc5ac production, as well as BALF and lung oxidative stress markers at least comparably and in many cases more than CS. BALF protein content at both time points studied was only elevated in the PG:VG-N + F group. After 3 days, PG: VG-Sol altered tissue elasticity, static compliance, and airway resistance, whereas after 4 wk CS was the only treatment adversely affecting these parameters. Airway hyperresponsiveness in response to methacholine was increased similarly in the CS and PG:VG-N + F groups. Our findings suggest that exposure to e-cig vapor can trigger inflammatory responses and adversely affect respiratory system mechanics. In many cases, the added flavor in e-cigs exacerbated the detrimental effects of e-cig vapor. We conclude that both e-cig vaping and conventional cigarette smoking negatively impact lung biology.