Technology used to build and transfer mammalian chromosomes
Technology used to build and transfer mammalian chromosomes
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DOI：
10.1016/j.yexcr.2020.111851
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发表时间：
2020-03-15
影响因子：
3.7
通讯作者：
Glass, John, I
中科院分区：
文献类型：
--
作者：
Brown, David M.;Glass, John, I
在人类和哺乳动物人工染色体领域的近二十年中，人造染色体构建和安装到所需的细胞类型或生物中的技术随着现代分子和合成生物学的其余发展而发展。医学，工业，制药，农业和基础研究科学家寻求人类和哺乳动物人工染色体的尚未实现的承诺。自上而下和自下而上的方法，用于构建这些人工染色体用于高等真核生物的技术非常不同，但渴望获得相似的结果。染色体大小合成DNA产生的新能力可能会将该领域转向更多自下而上的方法，但不是完全。同样，在靶细胞中安装人造人工染色体的新方法将与当前主导该领域的Microcell介导的细胞转移方法竞争。
In the near twenty-year existence of the human and mammalian artificial chromosome field, the technologies for artificial chromosome construction and installation into desired cell types or organisms have evolved with the rest of modem molecular and synthetic biology. Medical, industrial, pharmaceutical, agricultural, and basic research scientists seek the as yet unrealized promise of human and mammalian artificial chromosomes. Existing technologies for both top-down and bottom-up approaches to construct these artificial chromosomes for use in higher eukaryotes are very different but aspire to achieve similar results. New capacity for production of chromosome sized synthetic DNA will likely shift the field towards more bottom-up approaches, but not completely. Similarly, new approaches to install human and mammalian artificial chromosomes in target cells will compete with the microcell mediated cell transfer methods that currently dominate the field.