Transcriptome analyses of the Dof-like gene family in grapevine reveal its involvement in berry, flower and seed development.
Transcriptome analyses of the Dof-like gene family in grapevine reveal its involvement in berry, flower and seed development.
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DOI：
10.1038/hortres.2016.42
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发表时间：
2016
影响因子：
8.7
通讯作者：
Revers, Luis Fernando
中科院分区：
文献类型：
--
作者：
da Silva, Danielle Costenaro;Falavigna, Vitor da Silveira;Fasoli, Marianna;Buffon, Vanessa;Porto, Diogo Denardi;Pappas, Georgios Joannis, Jr.;Pezzotti, Mario;Pasquali, Giancarlo;Revers, Luis Fernando
DOF(DNA-bindingWith One Finger)蛋白家族涉及一组植物转录因子，参与调节多种功能，如植物对胁迫、激素和光的反应、光敏色素信号和种子萌发。在这里，我们描述了葡萄(Vitis vinifera L.)中的DOF-like基因家族，它由25个编码DOF的基因组成。对VviDofL基因家族进行了广泛的电子特征分析。此外，采用基因芯片(Cv Corvina)和实时聚合酶链式反应(Cv Pinot Noir)分析相结合的方法，对整个基因家族在54个葡萄组织和器官中的表达进行了评估。将葡萄与拟南芥、番茄、杨树的序列以及其他物种中已描述的DOF基因进行系统发育分析，使我们能够识别出几个重复的基因。葡萄DofL基因在进化过程中的多样性可能导致了更广泛的生物学作用。此外，在被分析的样本之间发现了不同的表达模式，证实了这一假设。我们的表达结果表明，几个VviDofL基因主要在花、果实和种子发育过程中发挥功能，这突显了它们对葡萄生长和生产的重要性。这两种方法之间相似的表达谱的鉴定有力地表明，这些基因具有重要的调节作用，在进化上在葡萄CVS Corvina和黑比诺之间是保守的。
The Dof (DNA-binding with one finger) protein family spans a group of plant transcription factors involved in the regulation of several functions, such as plant responses to stress, hormones and light, phytochrome signaling and seed germination. Here we describe the Dof-like gene family in grapevine (Vitis vinifera L.), which consists of 25 genes coding for Dof. An extensive in silico characterization of the VviDofL gene family was performed. Additionally, the expression of the entire gene family was assessed in 54 grapevine tissues and organs using an integrated approach with microarray (cv Corvina) and real-time PCR (cv Pinot Noir) analyses. The phylogenetic analysis comparing grapevine sequences with those of Arabidopsis, tomato, poplar and already described Dof genes in other species allowed us to identify several duplicated genes. The diversification of grapevine DofL genes during evolution likely resulted in a broader range of biological roles. Furthermore, distinct expression patterns were identified between samples analyzed, corroborating such hypothesis. Our expression results indicate that several VviDofL genes perform their functional roles mainly during flower, berry and seed development, highlighting their importance for grapevine growth and production. The identification of similar expression profiles between both approaches strongly suggests that these genes have important regulatory roles that are evolutionally conserved between grapevine cvs Corvina and Pinot Noir.
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影响因子：
10.7
作者：
Castresana, J
通讯作者：
Castresana, J
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作者：
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Gabriele, Stefano;Rizza, Annalisa;Vittorioso, Paola
通讯作者：
Vittorioso, Paola
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64.8
作者：
Jaillon, Olivier;Aury, Jean-Marc;Wincker, Patrick
通讯作者：
Wincker, Patrick
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5.1
作者：
Dong, Guoqing;Ni, Zhongfu;Sun, Qixin
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