Expression of integrin alpha v beta 3 in small blood vessels of glioblastoma tumors
Expression of integrin alpha v beta 3 in small blood vessels of glioblastoma tumors
复制标题
DOI：
10.1097/00005072-199611000-00005
复制
发表时间：
1996-11-01
影响因子：
3.2
通讯作者：
Gladson, CL
中科院分区：
文献类型：
--
作者：
Gladson, CL
促进肿瘤增殖和侵袭的血管生成是由整合素依赖性细胞粘附机制介导的，需要整合素αvβ3的表达。为了确定整合素αvβ3是否在胶质母细胞瘤肿瘤的小血管增生特征中表达，我们探讨了整合素αv、β3、β5和β1亚基在不同级别和级别的胶质瘤小血管中的mRNA和蛋白表达。非肿瘤性脑活检。反义β3和β5核糖探针与11个胶质母细胞瘤中的11个和4个间变性星形细胞瘤中的2个中的小血管（内皮细胞和邻近间充质细胞）杂交，但未能与12个非肿瘤标本和4个低级别星形细胞瘤中的小血管杂交。相比之下，反义αv和β1核糖探针在所有活检中都与小血管杂交。使用抗整合素亚基特异性抗体通过免疫组织化学分析测定这些血管中整合素αvβ3蛋白的表达。结果与使用原位杂交获得的结果相似：在 12 例胶质母细胞瘤中的 9 例和 4 例间变性星形细胞瘤中的 2 例的小血管中检测到 β 3 亚基蛋白，而在 10 例非肿瘤性脑和 4 例低级别星形细胞瘤的小血管中未检测到β 3 亚基蛋白。相比之下，在所有脑活检的小血管中都检测到了 α v 和 β 1 亚基蛋白，而无法检测到 β 5 亚基蛋白。这些数据表明整合素αvβ3在胶质母细胞瘤谣言的小血管中表达。
Angiogenesis, which promotes tumor proliferation and invasiveness, is mediated by integrin-dependent cell adhesion mechanisms and requires expression of integrin alpha v beta 3. To determine whether integrin alpha v beta 3 is expressed in the small blood vessel hyperplasia characteristic of glioblastoma tumors, we explored the mRNA and protein expression of integrin alpha v, beta 3, beta 5, and beta 1 subunits in small blood vessels in gliomas of various grades and nontumorous brain biopsies. Antisense beta 3 and beta 5 riboprobes hybridized to small blood vessels (endothelial and adjacent mesenchymal cells) in 11 of 11 glioblastomas and 2 of 4 anaplastic astrocytomas, but failed to hybridize to small blood vessels in 12 non-neoplastic specimens and 4 low-grade astrocytomas. In contrast, antisense alpha v and beta 1 riboprobes hybridized to small blood vessels in all of the biopsies. The expression of integrin alpha v beta 3 protein in these vessels was determined by immunohistochemical analysis using anti-integrin subunit-specific antibodies. The findings were similar to those obtained using in situ hybridization: beta 3 subunit protein was detected on small blood vessels in 9 of 12 glioblastoma and 2 of 4 anaplastic astrocytomas, whereas it was not detected in small blood vessels of 10 non-neoplastic brains and 4 low-grade astrocytomas. In contrast, the alpha v and beta 1 subunit proteins were detected in small blood vessels in all of the brain biopsies, whereas the beta 5 subunit protein could not be detected. These data suggest that integrin alpha v beta 3 is expressed in small blood vessels of glioblastoma rumors.