Effects of mechanical stimuli on the synthesis of superficial zone protein in chondrocytes
Effects of mechanical stimuli on the synthesis of superficial zone protein in chondrocytes
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DOI：
10.1002/jbm.a.32295
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发表时间：
2010-02-01
影响因子：
4.9
通讯作者：
Tanne, K.
中科院分区：
文献类型：
--
作者：
Kamiya, T.;Tanimoto, K.;Tanne, K.
表面区蛋白(SZP)已被证明在滑膜关节的边界润滑中起作用。本研究旨在阐明机械应力对关节软骨细胞SZP表达的调节作用。将细胞伸长率分别为7%和21%的循环拉伸应变分别作用于培养的猪下颌骨软骨细胞。用实时荧光定量聚合酶链式反应检测SZP、IL-1β和TGF-β1的mRNA水平。Western blotting检测SZP蛋白水平。在12、24和4811伸长7%后，SZP的mRNA水平显著上调。虽然SZP的mRNA水平在12h后增加了21%，但在48h后下降到低于对照组的水平，而转化生长因子-β1mRNA水平的变化与SZP相似。7%的伸长率对IL-1βmRNA水平无明显影响。然而，施加21%的伸长率12h后，IL-1β的mRNA水平显著增加。Western印迹分析表明，SZP的表达增加了7%，但显著降低了21%的伸长。这些结果表明，SZP在软骨细胞中的表达水平在适宜的机械刺激下被增强，但在过度负荷下被抑制，部分受转化生长因子-β1和IL-1β的影响，从而导致关节润滑的恶化。(C)2009年Wiley期刊，Inc.《生物医学杂志》92A：801-805,2010
Superficial zone protein (SZP) has been demonstrated to contribute to the boundary lubrication in synovial joints. This study was designed to clarify the modulation of SZP expression by mechanical stress in articular chondrocytes. Cyclic tensile strains of 7 and 21% cell elongation were applied to cultured chondrocytes obtained from porcine mandibular condylar cartilage. The mRNA levels of SZP, IL-1 beta, and TGF-beta 1 were examined by a quantitative real-time PCR analysis. Protein level of SZP was examined by Western blotting. The SZP mRNA level was significantly upregulated after 12, 24, and 48 11 by 7% elongation. Although SZP mRNA level was upregulated by 21% elongation after 12 h, it decreased to a lower level than the control after 48 h. The TGF-beta 1 mRNA level exhibited an almost similar change to SZP. The IL-1 beta mRNA level was not changed markedly by 7% elongation. However, the IL-1 beta mRNA level was significantly increased by a 12-h application of 21% elongation. Western blot analysis revealed that the SZP expression was increased by 7% elongation, but decreased remarkably by 21% elongation. It is suggested from these findings that the SZP expression level in the chondrocytes is enhanced by optimal mechanical stimuli, but inhibited by excessive loading partly affected by TGF-beta 1. and IL-1 beta, leading to the deterioration of joint lubrication. (C) 2009 Wiley Periodicals, Inc. J Biomed Mater Res 92A: 801-805, 2010