Molecularly Imprinted Nanogels Possessing Dansylamide Interaction Sites for Controlling Protein Corona In Situ by Cloaking Intrinsic Human Serum Albumin
Molecularly Imprinted Nanogels Possessing Dansylamide Interaction Sites for Controlling Protein Corona In Situ by Cloaking Intrinsic Human Serum Albumin
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DOI：
10.1021/acs.langmuir.0c00927
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发表时间：
2020-09-15
期刊：
影响因子：
3.9
通讯作者：
Takeuchi, Toshifumi
中科院分区：
文献类型：
--
作者：
Morishita, Takahiro;Yoshida, Aoi;Takeuchi, Toshifumi
纳米材料由于其独特的生物学特性，在生物医学领域的应用前景日益广阔。作为药物递送载体，纳米材料必须在血流中循环以将封装的组分递送到靶组织。蛋白质冠调控是一种很有前途的方法，它可以提供隐形能力以避免免疫反应。本研究的目的是开发分子印迹聚合物纳米凝胶（MIP-NG）能够蛋白质电晕调节，使用固有的人血清白蛋白（HSA）和功能单体，丹磺酰胺乙基丙烯酰胺（DAEAm），丹磺酰胺基团作为配体的HSA。采用等温滴定量热法（ITC）研究了HSA对MIP-NG的识别能力。然后将用DAEAm制备的MIP-NG的亲和力与在我们先前的研究中开发的用丙烯酸吡咯烷酯制备的参考MIP-NG的亲和力进行比较。此外，我们证明，同时使用这两种不同的功能单体的分子印迹是进一步有效地构建高亲和力的识别HSA的纳米腔和形成富含HSA的蛋白质冠在人血浆中，由于不同的相互作用模式的单体。我们认为，通过使用基于配体的功能单体开发的分子印迹策略是创建人工分子识别材料的有效策略。
Nanomaterials have become increasingly promising for biomedical applications owing to their specific biological characteristics. As drug delivery vehicles, nanomaterials have to circulate in the bloodstream to deliver the encapsulated components to the target tissues. Protein corona regulation is one of the promising approaches that gives stealth capability to avoid immune response. The aim of this study was to develop molecularly imprinted polymer nanogels (MIP-NGs) capable of protein corona regulation, using intrinsic human serum albumin (HSA) and with a functional monomer, dansylamide ethyl acrylamide (DAEAm), the dansylamide group serving as a ligand for HSA. The recognition capability of HSA for MIP-NGs was investigated by isothermal titration calorimetry (ITC). The affinity of the MIP-NGs prepared with DAEAm was then compared to that of the reference MIP-NGs prepared with pyrrolidyl acrylate developed in our previous study. Furthermore, we demonstrated that the concurrent use of these two different functional monomers for molecular imprinting was further effective to construct high-affinity recognition nanocavities for HSA and to form HSA-rich protein corona in the human plasma owing to the different interaction modes of the monomers. We believe that the molecular imprinting strategy developed through the use of ligand-based functional monomer is an effective strategy to create artificial molecular recognition materials.