Exposure of cultured hepatocytes to cyclic AMP enhances the vasopressin-mediated stimulation of inositol phosphate production.
Exposure of cultured hepatocytes to cyclic AMP enhances the vasopressin-mediated stimulation of inositol phosphate production.
复制标题
将培养的肝细胞暴露于环磷酸腺苷会增强加压素介导的磷酸肌醇产生的刺激。
DOI：
10.1042/bj2570455
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发表时间：
1989
期刊：
影响因子：
--
通讯作者：
Fain,JN
中科院分区：
文献类型：
--
作者：
Pittner,RA;Fain,JN
分离的大鼠肝细胞在原代单层培养中，在10%(v/v)的血清和[~3H]肌醇存在下维持18-24小时。加压素(100 NM)刺激一、二、三磷酸肌醇(IP1、IP2和IP3)的产生。预先给予8-溴环腺苷(8BrcAMP；100微米)，而不是8-溴环GMP，可增强加压素介导的磷酸肌醇积累的刺激作用，但在没有加压素的情况下对其形成没有显著影响。用高血糖素(10 NM)模拟环状AMP类似物的作用，观察在加入加压素前5min或12h加入环状AMP或胰高血糖素。在8BR-cAMP和加压素存在的情况下，地塞米松(100 NM)孵育8h可增加IP3的积聚，但不增加IP2或IP1的积聚。有无8BrcAMP作用8h后，放线菌素D或放线菌素D对加压素刺激的肌醇磷酸蓄积作用无明显影响。
Isolated rat hepatocytes in primary monolayer culture were maintained for 18-24 h in the presence of 10% (v/v) serum and [3H]inositol. Vasopressin (100 nM) stimulated the production of inositol mono-, bis- and tris-phosphates (IP1, IP2, and IP3). Prior exposure of hepatocytes to 8-bromo cyclic AMP (8Br-cAMP; 100 microM), but not 8-bromo cyclic GMP, enhanced the vasopressin-mediated stimulation of inositol phosphate accumulation, but had no significant effect on their formation in the absence of vasopressin. The effect of the cyclic AMP analogue was mimicked by glucagon (10 nM), and was seen whether cyclic AMP or glucagon was added 5 min or 12 h before the addition of vasopressin. An 8 h incubation with dexamethasone (100 nM) enhanced the accumulation of IP3, but not that of IP2 or IP1, in the presence of 8Br-cAMP and vasopressin. Cycloheximide or actinomycin D had little effect on the vasopressin stimulation of inositol phosphate accumulation, after an 8 h incubation in the presence or absence of 8Br-cAMP.