Association between dependent smoking and a polymorphism in the tyrosine hydroxylase gene in a prospective population-based study of adolescent health
Association between dependent smoking and a polymorphism in the tyrosine hydroxylase gene in a prospective population-based study of adolescent health
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DOI：
10.1023/b:bege.0000009478.70863.25
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发表时间：
2004-01-01
影响因子：
2.6
通讯作者：
Williamson, R
中科院分区：
文献类型：
--
作者：
Olsson, C;Anney, R;Williamson, R
本研究报告了烟草依赖和酪氨酸羟化酶（TH）基因第一内含子中的五个等位基因四核苷酸重复多态性之间的关联的试点数据。126名澳大利亚青少年参加了过渡期健康研究（1993-1997年），并在四波数据收集中表现出依赖性或非依赖性吸烟模式，他们同意参加试点研究。在2000年期间，使用电话管理的药物使用调查表确认了被招募者的吸烟状况。烟草依赖定义为在第5波（2000年）和至少一个前波（n=58）期间每周吸烟超过6天，每天吸烟超过10支。第二个更严格的表型包括在清醒后一小时内吸烟（n=37）。对照组包括使用烟草但在每一波数据中保持低水平社交吸烟者的青少年（n=56）。使用漱口水程序收集DNA。使用更严格定义的烟草依赖表型，调整性别后，发现K4等位基因和烟草依赖之间存在显著的保护性关联（OR 0.27，95%置信区间[CI] 0.09，0.82）。使用烟草依赖的自由标准未发现相关性（OR 0.51，95%置信区间[CI] 0.23，1.2）。这些初步结果重复了烟草使用与TH基因的K4等位基因之间的先前关联（Lerman等人，1997年）。包括时间的烟草依赖的定义中的第一支香烟的潜在意义和可能的作用，这些TH变异体可能在烟草依赖中发挥的作用进行了讨论。
This study reports pilot data on an association between tobacco dependence and a five-allele tetranucleotide repeat polymorphism in the first intron of the tyrosine hydroxylase (TH) gene. One hundred and twenty-six Australian adolescents who had participated in the Health in Transition Study (1993-1997), and who showed patterns of either dependent or nondependent smoking across four waves of data collection, consented to participation in the pilot study. The smoking status of those recruited was confirmed using a telephone-administered drug use questionnaire during 2000. Tobacco dependence was defined as smoking more than 6 days per week and more than 10 cigarettes per day during wave 5 (year 2000) and at least one prior wave (n=58). A second, more stringent phenotype included smoking within an hour of waking (n=37). The control group comprised adolescents who had used tobacco but had remained low-level social smokers across each wave of data (n=56). DNA was collected using a mouthwash procedure. Using the more strictly defined tobacco dependence phenotype, and after adjusting for sex, a significant protective association was found between the K4 allele and tobacco dependence (OR 0.27, 95% confidence interval [CI] 0.09, 0.82). No association was found using the liberal criteria of tobacco dependence (OR 0.51, 95% confidence interval [CI] 0.23, 1.2). These preliminary results replicate a previous association between tobacco use and the K4 allele of the TH gene (Lerman et al., 1997). The potential significance of including time to first cigarette in definitions of tobacco dependence and the possible role that these TH variants might play in tobacco dependence are discussed.