Optimization of growth parameters for lipase production by Ganoderma lucidum using response surface methodology
Optimization of growth parameters for lipase production by Ganoderma lucidum using response surface methodology
复制标题
利用响应面法优化灵芝生产脂肪酶的生长参数
DOI：
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发表时间：
2011
期刊：
影响因子：
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通讯作者：
S. Rehman
中科院分区：
文献类型：
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作者：
Faiza Amin;H. Bhatti;S. Rehman
Lipases comprise a group of hydrolytic enzymes which catalyze reversibly the hydrolysis and synthesis of triacylglycerides in the oil water interface. In this study, a response surface approach was used to study the production of extracellular lipase from Ganoderma lucidum which has diverse applications in various fields. Interactions were studied for five different variables (moisture, canola oil cake, olive oil, pH and time of incubation) which were found influential for lipase production. Using the statistical approach (response surface methodology), the maximum yield of lipase (4838 U/gds) by G. lucidum was observed under optimum conditions. The optimum values of these parameters were as follows: canola oil cake (12.50 g), moisture level (60%), pH (4.5), olive oil as inducer (2.0%) and incubation period (96 h) at 30°C. Exploration of fungal diversity for improved production of lipases using statistical models makes it a high-profile area for novel discovery with enormous potential of massive returns. Key words : Lipases, Ganoderma lucidum, statistical approach, solid state fermentation, incubation period, canola oil cake.
