Pilot analysis of cytokines levels in stored granulocyte-colony-stimulating factor-mobilized peripheral blood stem cell concentrates.
Pilot analysis of cytokines levels in stored granulocyte-colony-stimulating factor-mobilized peripheral blood stem cell concentrates.
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对储存的粒细胞集落刺激因子动员的外周血干细胞浓缩物中细胞因子水平的初步分析。
DOI：
10.1111/j.1537-2995.2010.02695.x
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发表时间：
2010
期刊：
影响因子：
2.9
通讯作者：
Stroncek,DavidF
中科院分区：
文献类型：
--
作者：
Woods,Iyonna;Tawab-Amiri,Abdul;Byrne,Karen;Sabatino,Marianna;Stroncek,DavidF
背景：外周血干细胞(PBSC)浓缩物的输注有时与发热输血反应有关。研究设计与方法：9名健康受试者的等量粒细胞集落刺激因子(CSF)动员的PBSC浓缩液在2~8℃袋装保存48小时。采用巢式酶联免疫吸附试验检测PBSC浓缩后0、24、48h血浆中19种生长因子和生物反应调节剂的水平。结果：引起发热反应的外周血和血浆中可引起发热反应的可溶性白介素1β、白介素6和肿瘤坏死因子α水平无显著差异。PBSC中的转化生长因子-β-1、基质金属蛋白酶-8、CCL5/(活化后正常T细胞表达和分泌减少)和血小板衍生生长因子-AB的水平显著高于血浆，而CCL2/MCP-1的水平显著低于血浆。PBSC的保存时间对这五个因素的水平没有影响。IL-1β、IL-2、IL-7、IL-8、IL-12p70、IL-15、干扰素-γ、CD40L、粒细胞-巨噬细胞集落刺激因子水平有下降趋势，而肿瘤坏死因子-α和IL-10水平有升高趋势，但差异无统计学意义。
BACKGROUND:The transfusion of peripheral blood stem cell (PBSC) concentrates is sometimes associated with febrile transfusion reactions. PBSC concentrates contain large numbers of white blood cells, and during storage the levels of soluble cytokines that could cause transfusion reactions may increase.STUDY DESIGN AND METHODS:Aliquots of granulocyte–colony‐stimulating factor (CSF)–mobilized PBSC concentrates from nine healthy subjects were stored in bags at 2 to 8°C for 48 hours. The levels of 19 growth factors and biologic response modifiers were measured in the plasma of PBSC concentrates at 0, 24, and 48 hours of storage using a nested enzyme‐linked immunosorbent assay. The same 19 factor levels were also measured in blood plasma from six healthy subjects.RESULTS:There were no significant differences in the PBSC and plasma levels of soluble interleukin (IL)‐1β, IL‐6, and tumor necrosis factor (TNF)‐α, which can cause febrile reactions. The levels of TGF‐β1, matrix metalloproteinase‐8, CCL5/(reduced on activation normal T expressed and secreted), and platelet (PLT)‐derived growth factor‐AB were significantly greater in PBSCs than in plasma and the level of CCL2/MCP‐1 was significantly less in PBSCs. Duration of PBSC storage had no effect on the levels of these five factors. There was a trend for reduced levels of IL‐1β, IL‐2, IL‐7, IL‐8, IL‐12p70, IL‐15, interferon‐γ, CD40L, and granulocyte‐macrophage–CSF and increased levels of TNF‐α and IL‐10 levels in PBSC concentrates, but the differences were not significant.CONCLUSIONS:There was no increase in stored PBSC concentrates of cytokines that have been associated with febrile transfusion reactions; however, the levels of other factors that were likely released by PLTs and granulocytes during the collection process were elevated.