Lensfree holographic imaging for on-chip cytometry and diagnostics.
Lensfree holographic imaging for on-chip cytometry and diagnostics.
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DOI：
10.1039/b813943a
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发表时间：
2009-03-21
期刊：
影响因子：
6.1
通讯作者：
Ozcan A
中科院分区：
文献类型：
--
作者：
Seo S;Su TW;Tseng DK;Erlinger A;Ozcan A
我们在实验上展示了一种无透镜全息成像平台来执行芯片上的细胞术。通过控制光源的空间相干性，利用像素尺寸约为2µm的高分辨率传感器阵列，在芯片上记录每个细胞或微粒的二维全息衍射图。然后，通过使用定制开发的决策算法来处理记录的全息图像，以将检测到的全息图像纹理与现有的库图像进行匹配，以用于芯片上表征和对感兴趣的不同种类的解决方案进行计数。任何微观物体的全息衍射图都与同一物体的经典衍射图有很大不同。它提高了细胞图案的信噪比和信号的一致性；对于弱散射相对象(如小细菌或细胞)的芯片成像也显示出更好的灵敏度。我们通过自动表征红细胞、酵母细胞、大肠杆菌和不同大小的微粒的不同溶液，而无需使用任何透镜或显微镜物镜，验证了这种全息芯片上细胞术方法的显著改进。这种无透镜芯片上全息照相平台将特别适用于护理点式细胞术和诊断应用，例如涉及艾滋病毒或疟疾等传染病的应用。
We experimentally illustrate a lensfree holographic imaging platform to perform on-chip cytometry. By controlling the spatial coherence of the illumination source, we record a 2D holographic diffraction pattern of each cell or micro-particle on a chip using a high resolution sensor array that has ~2 µm pixel size. The recorded holographic image is then processed by using a custom developed decision algorithm for matching the detected hologram texture to existing library images for on-chip characterization and counting of a heterogeneous solution of interest. The holographic diffraction signature of any microscopic object is significantly different from the classical diffraction pattern of the same object. It improves the signal to noise ratio and the signature uniformity of the cell patterns; and also exhibits much better sensitivity for on-chip imaging of weakly scattering phase objects such as small bacteria or cells. We verify significantly improved performance of this holographic on-chip cytometry approach by automatically characterizing heterogeneous solutions of red blood cells, yeast cells, E. coli and various sized micro-particles without the use of any lenses or microscope objectives. This lensless on-chip holography platform will especially be useful for point-of-care cytometry and diagnostics applications involving e.g., infectious diseases such as HIV or malaria.