Arsenic exposure disrupts neurite growth and complexity in vitro
Arsenic exposure disrupts neurite growth and complexity in vitro
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DOI：
10.1016/j.neuro.2009.02.015
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发表时间：
2009-07-01
期刊：
影响因子：
3.4
通讯作者：
Currie, Douglas A.
中科院分区：
文献类型：
--
作者：
Frankel, Samuel;Concannon, John;Currie, Douglas A.
砷对成熟神经系统的中枢和外周成分都有神经毒性作用。越来越多的证据表明，接触砷对发育中的神经系统也有毒性，并可能导致培养的发育中的神经元细胞分裂减少和细胞凋亡增加。然而，砷对随后神经元生长和形态的影响尚不清楚。在本研究中，我们利用分化的PC12细胞来研究亚砷酸钠对早期神经突产生和生长的影响。我们发现砷对体外初始神经突生长具有浓度和时间依赖性。暴露于低微摩尔浓度的亚砷酸钠5天，可导致新分化的PC12细胞神经突的产生、生长和复杂性降低。此外，我们发现更成熟的PC12细胞暴露于亚砷酸盐可以抑制神经突的进一步发育。这些结果表明，暴露于砷可以通过改变形态发育的正常进程来破坏神经元分化的早期阶段，并可能导致神经元的长期功能受损。(C) 2009爱思唯尔公司版权所有。
Arsenic has neurotoxic effects on both central and peripheral components of the mature nervous system. There is increasing evidence that exposure to arsenic is also toxic to the developing nervous system and can result in decreased cell division and increased apoptosis in cultured developing neurons. However, the effects of arsenic on subsequent neuron growth and morphology remain unclear. In the present study we used differentiating PC12 cells to investigate the effects of sodium arsenite on the early stages of neurite production and growth. We find that arsenic has concentration- and time-dependent effects on initial neurite outgrowth in vitro. Exposure to low micromolar levels of sodium arsenite for five days results in reduced neurite production, outgrowth and complexity in newly differentiating PC12 cells. Furthermore, we find that exposure of more mature PC12 cells to arsenite can inhibit further neurite development. These results suggest that exposure to arsenic can disrupt early stages of neuron differentiation by altering the normal progression of morphological development and could potentially contribute to compromised long term functioning of neurons. (C) 2009 Elsevier Inc. All rights reserved.