Effect of Linolenic acid during in vitro maturation of ovine oocytes: embryonic developmental potential and mRNA abundances of genes involved in apoptosis
Effect of Linolenic acid during in vitro maturation of ovine oocytes: embryonic developmental potential and mRNA abundances of genes involved in apoptosis
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亚麻酸对绵羊卵母细胞体外成熟的影响：胚胎发育潜力和细胞凋亡相关基因的 mRNA 丰度
DOI：
10.1007/s10815-015-0439-9
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发表时间：
2015
影响因子：
3.1
通讯作者：
M. Çınar
中科院分区：
文献类型：
--
作者：
A. Veshkini;H. Asadi;A. Khadem;A. Mohammadi;Sina Khazabi;M. Aminafshar;H. Deldar;M. Soleimani;M. Çınar
目的研究α-亚麻酸(ALA)对绵羊卵母细胞减数分裂成熟、凋亡相关分子(BAX和Bcl2)基因丰度及囊胚形成的影响。方法采用气相色谱-质谱仪对绵羊卵母细胞“小”卵泡(≤2 mm)和“大”卵泡(≥6 0 mm)中ALA的浓度进行初步分析。小卵泡和大卵泡中丙氨酸的浓度分别为75.4~125.7μM。然后用0(对照组)、10(ALA-10)、50(ALA-50)、100(ALA-100)和200(ALA-200)μM进行卵母细胞体外成熟。在体外培养24小时后检测Bax、Bax和Bcl2基因的成熟程度和mRNA丰度。结果与对照组相比，最高浓度的ALA(ALA-200)降低了卵母细胞成熟率。体外成熟后卵母细胞的凋亡相关基因分析显示，与对照组相比，ALA处理组卵母细胞中Bax基因的转录丰度降低，而Bcl2基因的转录丰度升高。在卵裂率(被认为是2-细胞进展)方面，我们没有观察到组之间的任何差异。与对照组相比，ALA-100处理组的囊胚形成率明显增加。结论在体外成熟过程中，ALA处理通过增加囊胚的形成而提高了绵羊卵母细胞的发育能力，这可能与细胞凋亡调控基因的丰度改变有关。
PurposeTo study the effect of α-linolenic acid (ALA) on meiotic maturation, mRNA abundance of apoptosis-related (Bax and Bcl-2) molecules, and blastocyst formation in ovine oocytes.MethodsA preliminary experiment was conducted to analyze the concentration of ALA in “small” (≤2 mm) and “large” (≥6 mm) follicles using gas chromatography/mass spectrometry analysis. The concentration of ALA in small and large follicles was determined to be in a range of 75.4 to 125.7 μM, respectively. In vitro maturation (IVM) of oocyte was then performed in presence of 0 (control), 10 (ALA-10), 50 (ALA-50), 100 (ALA-100), and 200 (ALA-200) μM of ALA. Meiotic maturation and mRNA abundance of Bax, and Bcl-2 genes was evaluated after 24 h of IVM. The embryonic cleavage and blastocyst formation following parthenogenetic activation were also determined for each group.ResultsThe highest concentration of ALA (ALA-200) decreased the oocyte maturation rate compared with the control group. Analysis of apoptosis-related genes in oocytes after IVM revealed lesser transcript abundances for Bax gene, and higher transcript abundances for Bcl-2 gene in ALA-treated oocytes as compared with the control oocytes. In term of cleavage rate (considered as 2-cell progression), we did not observe any differences among the groups. However, ALA-100 group promoted more blastocyst formation as compared with the control group.ConclusionOur results suggested that ALA treatment during IVM had a beneficial effect on developmental competence of ovine oocytes by increasing the blastocyst formation and this might be due to the altered abundance of apoptosis-regulatory genes.
DOI：
10.1073/pnas.180295197
发表时间：
2000-08-29
影响因子：
11.1
作者：
Elvin, JA;Yan, CN;Matzuk, MM
通讯作者：
Matzuk, MM