Light acclimation interacts with thylakoid ion transport to govern the dynamics of photosynthesis in Arabidopsis
Light acclimation interacts with thylakoid ion transport to govern the dynamics of photosynthesis in Arabidopsis
复制标题
光驯化与类囊体离子运输相互作用，控制拟南芥光合作用的动态
DOI：
10.1111/nph.18534
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发表时间：
2023
期刊：
影响因子：
9.4
通讯作者：
Eirich, Jürgen
中科院分区：
文献类型：
--
作者：
von Bismarck, Thekla;Korkmaz, Kübra;Ruß, Jeremy;Skurk, Kira;Kaiser, Elias;Correa Galvis, Viviana;Cruz, Jeffrey A.;Strand, Deserah D.;Köhl, Karin;Eirich, Jürgen
了解自然，动态光环境中的光合作用需要了解长期适应，短期反应及其机械相互作用。为了探讨后者，我们系统地确定和表征了光环境对类囊体离子转运介导的短期响应的影响。为此，将拟南芥野生型和Cl−通道VCCN 1和K+交换反向转运蛋白KEA3的突变体在8种不同的光环境下生长，并在稳态和光波动期间表征了光合作用相关参数和因子。为了详细描述所选光照条件，我们通过改进的光谱方法以前所未有的高时间分辨率监测离子通量动态。我们的分析表明，每日光照强度塑造光合能力作为主要的驯化驱动因素，分别对高光阶段KEA3和VCCN 1的功能产生积极和消极影响。光强度的波动促进了光保护色素玉米黄质（Zx）的积累。我们发现KEA3抑制白天Zx的积累，这与其直接的质子运输活性一起加速光合作用向低光强的转变。总之，光环境因素，强度和变异性，调节动态光合作用中类囊体离子运输的功能，对管腔pH，Zx积累，光保护和光合效率有明显的影响。
Understanding photosynthesis in natural, dynamic light environments requires knowledge of long‐term acclimation, short‐term responses, and their mechanistic interactions. To approach the latter, we systematically determined and characterized light‐environmental effects on thylakoid ion transport‐mediated short‐term responses during light fluctuations.For this,Arabidopsis thalianawild‐type and mutants of the Cl−channel VCCN1 and the K+exchange antiporter KEA3 were grown under eight different light environments and characterized for photosynthesis‐associated parameters and factors in steady state and during light fluctuations. For a detailed characterization of selected light conditions, we monitored ion flux dynamics at unprecedented high temporal resolution by a modified spectroscopy approach.Our analyses reveal that daily light intensity sculpts photosynthetic capacity as a main acclimatory driver with positive and negative effects on the function of KEA3 and VCCN1 during high‐light phases, respectively. Fluctuations in light intensity boost the accumulation of the photoprotective pigment zeaxanthin (Zx). We show that KEA3 suppresses Zx accumulation during the day, which together with its direct proton transport activity accelerates photosynthetic transition to lower light intensities.In summary, both light‐environment factors, intensity and variability, modulate the function of thylakoid ion transport in dynamic photosynthesis with distinct effects on lumen pH, Zx accumulation, photoprotection, and photosynthetic efficiency.