The significance of antilymphocyte antibodies in patients with acquired immune deficiency syndrome (AIDS) and their sexual partners.
The significance of antilymphocyte antibodies in patients with acquired immune deficiency syndrome (AIDS) and their sexual partners.
复制标题
抗淋巴细胞抗体对获得性免疫缺陷综合征（艾滋病）患者及其性伴侣的意义。
DOI：
10.1007/bf00916007
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发表时间：
1987
影响因子：
9.1
通讯作者：
Kirkpatrick,CH
中科院分区：
文献类型：
--
作者：
Ozturk,GE;Kohler,PF;HorsburghJr,CR;Kirkpatrick,CH
抗淋巴细胞抗体已经在自身免疫性疾病、急性病毒感染和获得性免疫缺陷综合征(AIDS)中被证明是通过使用传统的微量淋巴细胞毒或双重荧光技术而被证明的。在本研究中，我们用这两种方法检测了艾滋病患者及其性伴的抗淋巴细胞抗体，并进一步探讨了它们的免疫学意义。常规微量淋巴细胞毒试验结果显示，10例艾滋病患者中有8例和10例配偶中有6例有明显的抗淋巴细胞抗体水平，在寒冷和温暖的温度下，抗淋巴细胞抗体与B细胞和T细胞反应。被B、T、Daudi细胞和金黄色葡萄球菌吸收后抗体活性显著降低，但不是针对血小板或红细胞，这表明这些抗体不是针对HLAI类抗原，而是针对两组淋巴细胞共同的抗原。在患者中，抗淋巴细胞抗体与淋巴细胞减少症密切相关，但在伴侣中则不然。用C6缺乏血清作为补体的非裂解来源，用双荧光染色技术检测针对淋巴细胞亚类[辅助者(T4)和抑制者(T8)]的抗体，证明抗体与靶细胞结合，而不是像微量淋巴细胞毒法那样裂解靶细胞。结果表明，抗体是针对两种人群的，外周血中T4、T8细胞绝对数与抗体结合率之间没有相关性或关联性。综上所述，至少有两种抗淋巴细胞抗体：用常规微量淋巴细胞毒试验检测的淋巴细胞毒抗体和用双荧光染色技术检测的非细胞毒抗体。前者可能是淋巴细胞减少症的部分原因。后者可能改变淋巴细胞的功能。有抗淋巴细胞抗体的患者和伴侣也有抗HTLV-III抗体，尽管效价之间没有任何密切的相关性。这些发现支持这样一种可能性，即这两种类型的抗体都是作为全身免疫反应的一部分出现的，可能是由相同的病毒剂刺激的。
Antilymphocyte antibodies have been demonstrated in autoimmune diseases, acute viral infections, and acquired immune deficiency syndrome (AIDS) by using either the conventional microlymphocytotoxicity or the double fluorescence technique. In the present study, we used both methods to detect the antilymphocyte antibodies and to characterize further their immunologic significance in patients with AIDS and their sexual partners. The results using the conventional microlymphocytotoxicity method demonstrated that 8 of 10 patients with AIDS and 6 of 10 partners had significant levels of antilymphocyte antibodies which were reactive with B and T cells at cold and warm temperatures. A significant loss in antibody activity following absorption with B, T, and Daudi cells andStaphylococcus aureus,but not platelets or red cells, indicated that these antibodies are not directed to HLA class I antigens but, rather, to antigens that are common to both groups of lymphocytes. There is a close association between antilymphocyte antibodies and lymphopenia in patients but not in partners. Antibodies against lymphocyte subclasses [helper (T4) and suppressor (T8)] were detected by the double fluorescence staining technique, which employs C6-deficient serum as a nonlytic source of complement, and demonstrated the binding of antibodies to target cells, in contrast to lysing of the target cells as in the microlymphocytotoxicity method. The results of this assay showed that antibodies were directed to both populations, and there was no correlation or association between the absolute numbers of peripheral T4 and T8 cells and the percentage of antibody binding. Taken together, there appear to be at least two kinds of antilymphocyte antibodies: lymphocytotoxic antibodies detected by the conventional microlymphocytotoxicity assay and noncytotoxic antibodies detected by the double fluorescence staining technique. The former may be responsible in part for the lymphopenia. The latter may alter lymphocyte function. The patients and partners who had antilymphocyte antibodies also had anti-HTLV-III antibodies, although there was not any close correlation between titers. These findings support the possibility that both types of antibodies occur as part of a generalized immune response, possibly stimulated by the same viral agent.