DNA content of the B chromosomes in grasshopper Podisma kanoi Storozh.: (Orthoptera, Acrididae)
DNA content of the B chromosomes in grasshopper Podisma kanoi Storozh.: (Orthoptera, Acrididae)
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DOI：
10.1007/s10577-007-1128-z
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发表时间：
2007-04-01
影响因子：
2.6
通讯作者：
Rubtsov, Nikolay B.
中科院分区：
文献类型：
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作者：
Bugrov, Alexander G.;Karamysheva, Tatiana V.;Rubtsov, Nikolay B.
利用染色体显微切割技术获得了鹿野足蝗B染色体DNA文库。从显微切割的DNA文库中共分离到153个DNA克隆。其中20个被测序。将B染色体DNA序列与来自DDBJ/GenBank/EMBL数据库（http：//www.ddbj.nig.ac.jp/）的其他物种的序列进行比较。用标记的克隆DNA片段进行FISH后，观察到不同的信号模式。FISH信号与克隆的DNA片段描绘整个Bs或其不同的区域。一些克隆还在A染色体的着丝粒周围区域发出信号。其他克隆的DNA片段仅在A和B染色体上产生背景样信号。用来自这两个物种的B染色体的DNA探针对鹿野足蝗和札幌足蝗的B染色体进行比较FISH分析，发现在鹿野足蝗的B染色体的着丝粒周围和端粒区域内存在同源DNA。与日本松的B染色体含有大量富含rDNA的区域不同，鹿野松的一条B染色体上仅检测到一小簇rDNA。这些数据强烈建议一个独立的起源B染色体在两个密切相关的足属物种。
A DNA library derived from the B chromosome of Podisma kanoi was obtained by chromosome microdissection. A total of 153 DNA clones were isolated from the microdissected DNA library. Twenty of them were sequenced. A comparison of B chromosome DNA sequences with sequences of other species from the DDBJ/GenBank/EMBL database (http://www.ddbj.nig.ac.jp/) was performed. Different patterns of signals were observed after FISH with labeled cloned DNA fragments. FISH signals with cloned DNA fragments painted either whole Bs or their different regions. Some clones also gave signals in pericentromeric regions of A chromosomes. Other cloned DNA fragments gave only background-like signals on A and B chromosomes. Comparative FISH analysis of B chromosomes in Podisma kanoi and P. sapporensis with DNA probes derived from the Bs of these species revealed homologous DNA that was confined within pericentromeric and telometric regions of the B chromosome in P. kanoi. In contrast to the B chromosomes in P. sapporensis containing large regions enriched with rDNA, only a small cluster of rDNA was detected in one of the examined B chromosomes in P. kanoi. The data strongly suggest an independent origin of B chromosomes in two closely related Podisma species.