Epidural Analgesia Prevents Endotoxin-Induced Gut Mucosal Injury in Rabbits
Epidural Analgesia Prevents Endotoxin-Induced Gut Mucosal Injury in Rabbits
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硬膜外镇痛可预防兔子内毒素引起的肠道粘膜损伤
DOI：
10.1213/01.ane.0000153863.95598.08
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发表时间：
2005
影响因子：
5.7
通讯作者：
J. Takeda
中科院分区：
文献类型：
--
作者：
Shizuko Kosugi;H. Morisaki;T. Satoh;K. Ai;Michiko Yamamoto;J. Soejima;R. Serita;Y. Kotake;A. Ishizaka;J. Takeda
本实验观察了硬膜外镇痛对内毒素引起的肠屏障功能改变的影响。将28只雄性家兔随机分为0.5%利多卡因组（E组）和生理盐水组（C组）。在整个研究期间，先静脉注射溶液（0.4 mL/kg），然后连续输注（0.1 mL · kg−1 · h−1）。在连续输注脂多糖（15 g · kg-1 · h-1）的情况下，测量平均动脉血压、粘膜内pH值和血浆血栓调节蛋白浓度。4 h时，E组与C组相比，平均动脉压较低（P < 0.05），粘膜内pH较高（P < 0.01），血液稀释程度更深（P < 0.05），而血浆血栓调节蛋白水平升高程度相似。E组回肠湿/干重比值较C组小，肠黏膜组织病理学损伤评分较C组明显减轻（P < 0.01）。在单独的一系列实验中（每组n = 10），E组的粘膜渗透性显著低于C组（P < 0.05）。总的来说，这些研究表明，尽管灌注压和动脉氧含量显着下降，硬膜外镇痛最大限度地减少内毒素诱导的功能和结构损伤的肠粘膜可能通过内皮非依赖性机制。
In the present study, we evaluated the effect of epidural analgesia on the alterations of gut barrier function elicited by endotoxin in rabbits. After the placement of an epidural catheter, 28 male rabbits were randomized into either 0.5% lidocaine (group E) or saline (group C) group. The solutions (0.4 mL/kg) were epidurally injected, followed by continuous infusion (0.1 mL · kg−1 · h−1) throughout the study period. Under a continuous infusion of lipopolysaccharide (15 &mgr;g · kg−1 · h−1), mean arterial blood pressure, intramucosal pH, and plasma thrombomodulin concentrations were measured. At 4 h, mean arterial blood pressure was lower (P < 0.05), intramucosal pH was higher (P < 0.01), and the progression of hemodilution more profound (P < 0.05) in group E versus group C, whereas plasma thrombomodulin levels were increased to a similar extent between the groups. With less wet-to-dry weight ratio of ileum, histopathological injury scores of gut mucosa were significantly less in group E versus group C (P < 0.01). In a separate series of experiments (n = 10 each group), mucosal permeability in group E was significantly less compared with group C (P < 0.05). Collectively, these studies showed that despite a significant decrease of perfusion pressure and arterial oxygen content, epidural analgesia minimized endotoxin-induced functional and structural injury of gut mucosa possibly through endothelium-independent mechanisms.
简单的肠梗阻会导致人体细菌移位。
DOI：
10.1001/archsurg.1989.01410060065013
发表时间：
1989
期刊：
Archives of surgery (Chicago, Ill. : 1960)
影响因子：
--
作者：
Deitch,EA
通讯作者：
Deitch,EA
影响因子：
2.2
作者：
Wang, WY;Smail, N;Chaudry, IH
通讯作者：
Chaudry, IH
检测兔 IL-8 和 MCP-1 的灵敏和特异性免疫测定：介导白细胞募集至肺部的细胞因子。
影响因子：
2.2
作者：
Kajikawa,O;Goodman,RB;Johnson2nd,MC;Konishi,K;Martin,TR
通讯作者：
Martin,TR