Molecular Threading and Tunable Molecular Recognition on DNA Origami Nanostructures
Molecular Threading and Tunable Molecular Recognition on DNA Origami Nanostructures
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DNA 折纸纳米结构上的分子线程和可调分子识别。
DOI：
10.1021/ja403863a
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发表时间：
2013-08-21
影响因子：
15
通讯作者：
Fan, Chunhai
中科院分区：
文献类型：
--
作者：
Wu, Na;Czajkowsky, Daniel M.;Fan, Chunhai
DNA折纸技术为组装纳米技术设备和在单分子水平上研究生化反应带来了巨大的希望。为此，必须在DNA折纸纳米结构上建立良好控制的功能材料与预定义位置的连接，以实现可靠的测量和多种应用。然而，折纸脚手架的双面性质在这方面显示出局限性。我们假设，通常使用的二维DNA折纸设计的孔足够大，可以通过单链DNA。因此，最初位于折纸一侧的足够长的单链DNA应该能够通过折纸上的孔“穿”到另一侧。通过使用带有图案化的生物素化单链DNA间隔物的折纸，并用原子力显微镜(AFM)成像监测链霉亲和素的结合，我们提供了明确的证据，表明位于一侧的生物素配体确实已经穿过另一侧。我们的发现揭示了一个以前被忽视的关键设计特征，它应该为先前的实验提供新的解释，并为构建具有新功能的折纸结构提供新的机会。
The DNA origami technology holds great promise for the assembly of nanoscopic technological devices and studies of biochemical reactions at the single-molecule level. For these, it is essential to establish well controlled attachment of functional materials to predefined sites on the DNA origami nanostructures for reliable measurements and versatile applications. However, the two-sided nature of the origami scaffold has shown limitations in this regard. We hypothesized that holes of the commonly used two-dimensional DNA origami designs are large enough for the passage of single-stranded (ss)-DNA. Sufficiently long ssDNA initially located on one side of the origami should thus be able to "thread" to the other side through the holes in the origami sheet. By using an origami sheet attached with patterned biotinylated ssDNA spacers and monitoring streptavidin binding with atomic force microscopic (AFM) imaging, we provide unambiguous evidence that the biotin ligands positioned on one side have indeed threaded through to the other side. Our finding reveals a previously overlooked critical design feature that should provide new interpretations to previous experiments and new opportunities for the construction of origami structures with new functional capabilities.