DETECTION AND ESTIMATION OF THE BARLEY PROLAMIN CONTENT OF BEER AND MALT TO ASSESS THEIR SUITABILITY FOR PATIENTS WITH CELIAC-DISEASE
DETECTION AND ESTIMATION OF THE BARLEY PROLAMIN CONTENT OF BEER AND MALT TO ASSESS THEIR SUITABILITY FOR PATIENTS WITH CELIAC-DISEASE
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DOI：
10.1016/0009-8981(90)90082-4
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发表时间：
1990-08-15
影响因子：
5
通讯作者：
CICLITIRA, PJ
中科院分区：
文献类型：
--
作者：
ELLIS, HJ;FREEDMAN, AR;CICLITIRA, PJ
评估了麦芽、啤酒、棕色啤酒、苦啤酒和黑啤酒的大麦醇溶蛋白（大麦醇溶蛋白）含量。对电泳分离的啤酒样品进行单克隆抗体免疫过氧化物酶染色，结果显示存在免疫反应性大麦醇溶蛋白。基于多克隆抗体的酶联免疫吸附测定 (ELISA) 显示，每克麦芽中含有 1.6 毫克大麦醇溶蛋白，每品脱啤酒中含有 1.5 毫克大麦醇溶蛋白。患有乳糜泻的患者应避免摄入啤酒和含有麦芽的食物，因为它们含有可定量的大麦醇溶蛋白，已知这种物质会加剧病情。
Malt, lager, brown ale, bitter and stout were assessed for their barley prolamin (hordein) content. Monoclonal antibody immunoperoxidase staining of electrophoretically separated samples of beer revealed the presence of immunoreactive hordein. Polyclonal antibody based enzyme linked immunosorbant assays (ELISAs) revealed 1.6 mg of hordein per gram of malt and 1.5 mg per pint in the beers. Patients with coeliac disease should avoid ingestion of beer and foods that contain malt since they contain quantifiable amounts of hordein that is known to exacerbate the conditions.