A novel ring-like complex of Xenopus proteins essential for the initiation of DNA replication
A novel ring-like complex of Xenopus proteins essential for the initiation of DNA replication
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DOI：
10.1101/gad.1070003
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发表时间：
2003-05-01
影响因子：
10.5
通讯作者：
Takisawa, H
中科院分区：
文献类型：
--
作者：
Kubota, Y;Takase, Y;Takisawa, H
我们已经确定了芽殖酵母Sld5及其三个相互作用的蛋白质的爪蟾同源物。这些形成了一个新的复杂的启动DNA复制在非洲爪蟾卵提取物。该复合物以依赖于复制许可和S期CDK的方式与染色质结合。染色质结合的复合物和Cdc45是相互依赖的，这两个绑定需要非洲爪蟾Cut5，酵母同源物与Sld5相互作用。在复制染色质的复合物相互作用与Cdc45和MCM，复制机制的假定组件。电子显微镜进一步揭示了配合物具有环状结构。这些结果表明，该复合物在DNA复制的延伸阶段以及起始阶段起着至关重要的作用。
We have identified Xenopus homologs of the budding yeast Sld5 and its three interacting proteins. These form a novel complex essential for the initiation of DNA replication in Xenopus egg extracts. The complex binds to chromatin in a manner dependent on replication licensing and S-phase CDK. The chromatin binding of the complex and that of Cdc45 are mutually dependent and both bindings require Xenopus Cut5, the yeast homolog of which interacts with Sld5. On replicating chromatin the complex interacts with Cdc45 and MCM, putative components of replication machinery. Electron microscopy further reveals that the complex has a ring-like structure. These results suggest that the complex plays an essential role in the elongation stage of DNA replication as well as the initiation stage.