Tunable somatosensory stimulation for selective sleep restriction studies in rodents.
Tunable somatosensory stimulation for selective sleep restriction studies in rodents.
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用于啮齿类动物选择性睡眠限制研究的可调节体感刺激。
DOI：
10.1109/embc.2016.7591028
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发表时间：
2016
期刊：
影响因子：
--
通讯作者：
Sunderam,Sridhar
中科院分区：
文献类型：
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作者：
Huffman,DillonM;Staggs,KendraE;Yaghouby,Farid;Agarwal,Anuj;O'Hara,BruceF;Donohue,KevinD;Blalock,EricM;Sunderam,Sridhar
已经出现了许多限制啮齿动物睡眠的方法，但大多数都是侵入性的，缺乏精细的控制，并引起压力。因此，一种多功能、非侵入性的睡眠限制方法，可以以可控的方式改变睡眠，这在睡眠研究中可能具有很大的价值。在之前的工作中，我们提出了一种基于机械振动的闭环体感刺激新系统，并将其应用于限制小鼠快速眼动（REM）睡眠的任务[1]。虽然这个系统是有效的，但它是一个粗糙的原型，不能精确控制施加在动物身上的刺激的幅度和频率。本文详细介绍了这个系统的进展，从一个二进制的，“全或无”的版本，允许动态控制扰动完成分级，状态依赖的睡眠限制。它的初步用途在两个应用中描述：大鼠的深度睡眠限制和小鼠的REM睡眠限制。
Many methods for sleep restriction in rodents have emerged, but most are intrusive, lack fine control, and induce stress. Therefore, a versatile, non-intrusive means of sleep restriction that can alter sleep in a controlled manner could be of great value in sleep research. In previous work, we proposed a novel system for closed-loop somatosensory stimulation based on mechanical vibration and applied it to the task of restricting Rapid Eye Movement (REM) sleep in mice [1]. While this system was effective, it was a crude prototype and did not allow precise control over the amplitude and frequency of stimulation applied to the animal. This paper details the progression of this system from a binary, “all-or-none” version to one that allows dynamic control over perturbation to accomplish graded, state-dependent sleep restriction. Its preliminary use is described in two applications: deep sleep restriction in rats, and REM sleep restriction in mice.