Identification of genes expressed in the epithelium of porcine oviduct containing early embryos at various stages of development
Identification of genes expressed in the epithelium of porcine oviduct containing early embryos at various stages of development
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DOI：
10.1002/1098-2795(200007)56:3
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发表时间：
2000-07-01
影响因子：
2.5
通讯作者：
Choo, KB
中科院分区：
文献类型：
--
作者：
Chang, HS;Cheng, WTK;Choo, KB
作为阐明输卵管上皮分泌因子作用的第一步，本研究使用差异显示逆转录聚合酶链反应（DDRT-PCR）来鉴定激素诱导排卵后携带早期胚胎发育各个阶段的礼物中此类输卵管因子的转录本。在 hCG 注射后 50 至 120 小时之间（1 细胞和 8 细胞胚胎阶段），总共鉴定出 13 个差异表达转录物。发现其中 12 个转录物最初在 hCG 注射后 96 小时（4 细胞胚胎阶段）及之后表达。序列分析显示其中的三个基因是猪转化生长因子-α、猪转化生长因子-β结合蛋白II和猪星形钠尿因子受体样转录物。 hCG注射后50-60和85小时之间仅检测到一个差异表达基因，该基因被证明是猪卵泡刺激素受体。 DDRT-PCR 检测到的其余 8 个转录本是新的。此外，发现大多数这些新表达的基因的开启时间与体外培养的猪胚胎4细胞块的开启时间一致。我们的结果表明，DDRT-PCR 是快速鉴定输卵管上皮差异表达基因的可行方法。由此鉴定出的一些基因可能对于输卵管中胚胎的不受阻碍的发育很重要。摩尔。重现。开发。 56:331-335, 2000。(C) 2000 Wiley-Liss, Inc.
As a first step toward elucidation of the action of factors secreted by the epithelium of oviduct, differential display reverse transcription-polymerase chain reaction (DDRT-PCR) was used in this study to identify transcripts of such oviductal factors in gifts carrying various stages of early embryo development post hormone-induced ovulation. A total of 13 differentially expressed transcripts were identified between 50 and 120 hr post-hCG injection (between 1-and 8-cell embryonic stages). Twelve of these transcripts were found to be initially expressed at 96 hr post-hCG injection (at 4-cell embryonic stage) and beyond. Three of such genes were shown by sequence analysis to be the porcine transforming growth factor-alpha, the porcine transforming growth factor-beta-binding protein II and a porcine astral natriuretic factor receptorlike transcript. Only one differentially expressed gene was detected between 50-60 and 85 hr post-hCG injection, and this gene turned out to be the porcine follicle-stimulating hormone receptor. The remaining eight transcripts detected by DDRT-PCR were novel. Moreover, most of these newly expressed genes were found to be turned on at a time coincidental with that of the 4-cell block of porcine embryos cultured in vitro. Our results demonstrate that DDRT-PCR is a feasible approach for rapid identification of genes that are differentially expressed in oviductal epithelium. Some of the genes thus identified may be important for unhindered development of embryos in the oviduct. Mol. Reprod. Dev. 56:331-335, 2000. (C) 2000 Wiley-Liss, Inc.