Dynamics of the antibody response in patients with therapeutic or spontaneous cure of American cutaneous leishmaniasis
Dynamics of the antibody response in patients with therapeutic or spontaneous cure of American cutaneous leishmaniasis
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DOI：
10.1016/s0035-9203(01)90168-3
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发表时间：
2001-03-01
影响因子：
2.2
通讯作者：
Abath, FGC
中科院分区：
文献类型：
--
作者：
Brito, MEF;Mendonça, MG;Abath, FGC
在Perambuco Stare，巴西，在1995年至1996年收集的血清中的抗原特异性和抗体反应的水平进行了分析，从58例临床美国皮肤利什曼病（ACL），25例ACL患者与锑酸葡甲胺化疗后明显治愈，和10 ACL患者自发治愈。评估是通过免疫印迹分析，ELISA和间接免疫荧光，与利什曼原虫（Viannia）巴西抗原，特别是在评估抗体反应的动态是否可以用于监测临床治愈的兴趣。IgG抗体反应性的明显下降，注意到临床愈合后，所有的抗原分析，除了19 kDa的抗原，其识别频率实际上增加了自发治愈的患者，这表明该抗原可能发挥作用，在保护性免疫对皮肤利什曼病。在临床愈合的患者中，最常识别的抗原（27和30 kDa抗原）的识别频率降低了约2倍，这表明它们可用作ACL治愈的标志物。此外，生活在流行地区的一些健康个体呈现出与活动性ACL中观察到的相同的免疫印迹反应模式，可能代表无症状感染个体。
The antigen specificity and the level of the antibody response were analysed in Perambuco Stare, Brazil, in sera collected in 1995-96 from 58 patients with clinical American cutaneous leishmaniasis (ACL), 25 ACL patients with apparent cure after chemotherapy with meglumine antimonate, and 10 ACL patients with spontaneous cure. Assessment was by immunoblot analysis, ELISA and indirect immunofluorescence, with Leishmania (Viannia) braziliensis antigens, with a particular interest in evaluating whether the dynamics of the antibody response could be useful to monitor clinical cure. A clear decrease of IgG antibody reactivity was noticed after clinical healing, for all of the antigens analysed, with the exception of the 19 kDa antigen, whose recognition frequency in fact increased in the spontaneously cured patients, suggesting that this antigen may play a role in protective immunity against cutaneous leishmaniasis. The recognition frequencies of the most frequently recognized antigens (27 and 30 kDa antigens) diminished approximately 2-fold in patients clinically healed, suggesting that they could be useful as a marker of cure of ACL. In addition, some of the healthy individuals living in endemic areas presented the same immunoblotting pattern of reactivity observed in active ACL, possibly representing asymptomatically infected individuals.