Circulating miRNA-122 levels are associated with hepatic necroinflammation and portal hypertension in HIV/HCV coinfection.
Circulating miRNA-122 levels are associated with hepatic necroinflammation and portal hypertension in HIV/HCV coinfection.
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DOI：
10.1371/journal.pone.0116768
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发表时间：
2015
期刊：
影响因子：
3.7
通讯作者：
Trebicka J
中科院分区：
文献类型：
--
作者：
Jansen C;Reiberger T;Huang J;Eischeid H;Schierwagen R;Mandorfer M;Anadol E;Schwabl P;Schwarze-Zander C;Warnecke-Eberz U;Strassburg CP;Rockstroh JK;Peck-Radosavljevic M;Odenthal M;Trebicka J
联合抗逆转录病毒治疗（cART）的引入改善了HIV感染者的生存率，而肝脏相关死亡率的相对贡献增加了。特别是在HIV/HCV合并感染的患者中，肝纤维化和门静脉高压是肝脏相关疾病发病率和死亡率的主要原因。循环中的miRNA-122水平在HIV患者中升高，并已被证明与肝损伤的严重程度相关。然而，miRNA-122水平与肝纤维化和门脉高压症的关系在HIV/HCV合并感染中仍有待研究。共纳入74例（31%为女性）HIV/HCV合并感染患者。通过定量聚合酶链反应（PCR）分析miRNA-122的血清水平，并将其标准化为SV-40加标RNA。52例（70%）患者测量了肝静脉压力梯度（HVPG），63例（85%）患者使用瞬时弹性成像确定了纤维化分期。在HIV/HCV合并感染患者中，循环miRNA-122水平升高，并与丙氨酸氨基转移酶（ALT）（rs = 0.438; p<0.001）和天冬氨酸氨基转移酶AST值（rs = 0.336; p = 0.003）显著相关，但与纤维化分期无关（p = n.s.）。有趣的是，miRNA-122水平与肝静脉压力梯度（HVPG）呈负相关（rs =-0.302; p = 0.03）。miRNA-122水平升高与肝损伤和低HVPG相关。虽然miRNA-122水平不适合预测肝纤维化程度，但它们可能作为HIV/HCV合并感染患者门脉高压的指标。
Introduction of combined antiretroviral therapy (cART) has improved survival of HIV infected individuals, while the relative contribution of liver-related mortality increased. Especially in HIV/HCV-coinfected patients hepatic fibrosis and portal hypertension represent the main causes of liver-related morbidity and mortality. Circulating miRNA-122 levels are elevated in HIV patients and have been shown to correlate with severity of liver injury. However, the association of miRNA-122 levels and hepatic fibrosis and portal hypertension remains to be explored in HIV/HCV coinfection. From a total of 74 (31% female) patients with HIV/HCV coinfection were included. Serum levels of miRNA-122 were analyzed by quantitative polymerase chain reaction (PCR) and normalized to SV-40 spike-in RNA. Hepatic venous pressure gradient (HVPG) was measured in 52 (70%) patients and the fibrosis stage was determined in 63 (85%) patients using transient elastography. The levels of circulating miRNA-122 were increased in HIV/HCV coinfected patients and significantly correlated with the alanine aminotransferase (ALT) (rs = 0.438; p<0.001) and aspartate transaminase AST values (rs = 0.336; p = 0.003), but not with fibrosis stage (p = n.s.). Interestingly, miRNA-122 levels showed an inverse correlation with hepatic venous pressure gradient (HVPG) (rs = −0.302; p = 0.03). Elevated miRNA-122 levels are associated with liver injury, and with low HVPG. Though, miRNA-122 levels are not suitable to predict the degree of fibrosis, they might function as indicators for portal hypertension in HIV/HCV coinfected patients.
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