Increased cerebrospinal fluid concentrations of soluble Fas (CD95/Apo-1) in hydrocephalus
Increased cerebrospinal fluid concentrations of soluble Fas (CD95/Apo-1) in hydrocephalus
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DOI：
10.1136/adc.84.4.369
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发表时间：
2001-04-01
影响因子：
5.2
通讯作者：
Bührer, C
中科院分区：
文献类型：
--
作者：
Felderhoff-Mueser, U;Herold, R;Bührer, C
背景和目的——在患有慢性颅内压（ICP）的儿童中观察到的脑室扩大会导致脑组织的继发性损失。在脑积水的动物研究中，程序性细胞死亡（细胞凋亡）已被发现是神经元损伤的主要机制。细胞凋亡程序的调节因子之一是受体介导的Fas/Fas配体相互作用。方法-在31例接受症状性脑积水分流手术的脑积水儿童和18名对照的脑脊液（CSF）中研究细胞凋亡调节细胞因子可溶性Fas（sFas）和可溶性Fas配体（sFasL）。结果-在患有症状性脑积水的儿童中观察到高浓度的sFas。脑积水（中位数 252 ng/ml）；对照中 sFas 低于检测限 (0.5 ng/ml)。除一份样本外，所有样本均检测不到 sFasL。 结论：脑积水儿童脑脊液中高浓度的 sFas 表明存在内在 sFas 产生，可能拮抗压力介导的 Fas 激活。
Background and aims-The ventricular enlargement observed in children with chronically raised intracranial pressure (ICP) causes a secondary loss of brain tissue. In animal studies of hydrocephalus, programmed cell death (apoptosis) has been found as a major mechanism of neuronal injury. One of the regulators of the apoptotic cell death programme is the receptor mediated Fas/Fas ligand interaction.Methods-The apoptosis regulating cytokines soluble Fas (sFas) and soluble Fas ligand (sFasL) were studied in the cerebrospinal fluid (CSF) of 31 hydrocephalic children undergoing shunt surgery for symptomatic hydrocephalus and 18 controls.Results-High concentrations of sFas were observed in children with hydrocephalus (median 252 ng/ml); in controls sFas was below the detection limit (0.5 ng/ml). sFasL was undetectable in all but one sample.Conclusion-High concentrations of sFas in the CSF of children with hydrocephalus suggest intrinsic sFas production, potentially antagonising pressure mediated Fas activation.