Inflicting Controlled Nonthermal Damage to Subcellular Structures by Laser-Activated Gold Nanoparticles
Inflicting Controlled Nonthermal Damage to Subcellular Structures by Laser-Activated Gold Nanoparticles
复制标题
DOI：
10.1021/nl103142t
复制
发表时间：
2010-11-01
期刊：
影响因子：
10.8
通讯作者：
Volk, Martin
中科院分区：
文献类型：
--
作者：
Krpetic, Zeljka;Nativo, Paola;Volk, Martin
我们表明，低强度激光照射含有内体金纳米颗粒的癌细胞导致内体破裂和纳米颗粒逃逸到细胞质中，而不影响细胞的活力。我们的实验的低光强度允许我们排除光热效应作为潜在的机制，我们目前的结果表明，光生活性物种的光诱导自由基。这种非热机制也可能是重要的背景下，细胞死亡在较高的激光强度，这是以前的报道。
We show that low-intensity laser irradiation of cancer cells containing endosomal gold nanoparticles leads to endosome rupture and escape of the nanoparticles into the cytosol without affecting the cells' viability. The low light intensity of our experiments allows us to rule out photothermal effects as the underlying mechanism, and we present results that suggest photoinduced radicals as the photogenerated active species. This nonthermal mechanism may also he important in the context of cell death at higher laser intensities, which had been reported previously.