Multiphoton microscopy for structure identification in human prostate and periprostatic tissue: implications in prostate cancer surgery.
Multiphoton microscopy for structure identification in human prostate and periprostatic tissue: implications in prostate cancer surgery.
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DOI：
10.1111/j.1464-410x.2011.10169.x
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发表时间：
2011-11
影响因子：
4.5
通讯作者：
Mukherjee S
中科院分区：
文献类型：
--
作者：
Tewari AK;Shevchuk MM;Sterling J;Grover S;Herman M;Yadav R;Mudalair K;Srivastava A;Rubin MA;Zipfel WR;Maxfield FR;Xu C;Webb WW;Mukherjee S
为了测试多光子显微镜（MPM）是否可以通过与金标准组织病理学相似的放大率和分辨率来识别前列腺和前列腺周围结构。本研究包括95名机器人根治性直肠癌切除术患者，他们同意参加2007年开始的机构审查委员会批准的研究。用于成像的标本类型是切除的手术切缘和活检，以及从切除的前列腺获得的切片。用定制的MPM系统对标本进行成像。将所有图像与同一标本的苏木精/伊红组织病理学进行比较。新鲜切除、未处理和未染色组织的MPM可以识别所有相关的前列腺和前列腺周围结构，例如神经、血管、包膜、潜在腺泡以及病理变化，包括前列腺癌。这些结构和病理的组织学确认和相关性验证了MPM的发现。MPM显示出很大的希望，作为一种工具，实时手术中的组织病理学，而不需要切除或管理的造影剂。然而，结果将需要在真正的手术环境中使用微型MPM显微内窥镜进行确认。
To test whether multiphoton microscopy (MPM) might allow identification of prostatic and periprostatic structures with magnification and resolution similar to gold standard histopathology. The present study included 95 robotic radical prostatectomy patients who consented to participate in an Institutional Review Board-approved study starting in 2007. The types of specimens used for imaging were excised surgical margins and biopsies, and sections obtained from the excised prostate. The specimens were imaged with a custom-built MPM system. All images were compared with haematoxylin/eosin histopathology of the same specimen. MPM of freshly excised, unprocessed and unstained tissue can identify all relevant prostatic and periprostatic structures, such as nerves, blood vessels, capsule, underlying acini and also pathological changes, including prostate cancer. Histological confirmation and correlation of these structures and pathologies have validated the findings of MPM. MPM shows great promise as a tool for real-time intra-surgical histopathology without needing excision or administration of contrast agents. The results will, however, need to be confirmed in true surgical settings using a miniaturized MPM microendoscope.