THE HERPES-SIMPLEX VIRUS IMMEDIATE-EARLY PROTEIN, ICP4, IS REQUIRED TO POTENTIATE REPLICATION OF HUMAN IMMUNODEFICIENCY VIRUS IN CD4+ LYMPHOCYTES
THE HERPES-SIMPLEX VIRUS IMMEDIATE-EARLY PROTEIN, ICP4, IS REQUIRED TO POTENTIATE REPLICATION OF HUMAN IMMUNODEFICIENCY VIRUS IN CD4+ LYMPHOCYTES
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DOI：
10.1128/jvi.63.5.1861-1868.1989
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发表时间：
1989-05-01
影响因子：
5.4
通讯作者：
HAMMER, SM
中科院分区：
文献类型：
--
作者：
ALBRECHT, MA;DELUCA, NA;HAMMER, SM
在急性全病毒共感染系统中研究了人类免疫缺陷病毒（HIV）和单纯疱疹病毒（HSV）的相互作用。 CD4+淋巴CEM细胞被HIV-1感染，24小时后，被HSV-1（KOS株）或HSV突变体重复感染，这些突变体在指定立即早期转录调节蛋白ICP0、ICP4或ICP27的基因中具有确定的缺失。 KOS毒株、ICP0突变体和ICP27突变体证明了HIV复制的显着增强，但ICP4突变体没有证明，这表明ICP4对于这种作用是必需的，而ICP0和ICP27不是必需的。这些研究表明，HSV 可通过 HSV 调节蛋白 ICP4 的活性，成为共感染 CD4+ 细胞中 HIV 复制和基因表达的有效刺激剂。
The interaction of human immunodeficiency virus (HIV) and herpes simplex virus (HSV) was investigated in an acute whole-virus coinfection system. CD4+ lymphoid CEM cells were infected with HIV-1 and, 24 h later, superinfected with HSV-1 (strain KOS) or HSV mutants possessing defined deletions in genes specifying the immediate-early transcriptional regulatory proteins ICP0, ICP4, or ICP27. Marked potentiation of HIV replication was demonstrated with the KOS strain, the ICP0 mutant, and the ICP27 mutant, but not with the ICP4 mutant, indicating that ICP4 is essential and ICP0 and ICP27 are nonessential for this effect. These studies demonstrate that HSV can be a potent stimulator of HIV replication and gene expression in coinfected CD4+ cells through the activity of the HSV regulatory protein ICP4.